An inverse relationship between T cell receptor affinity and antigen dose during CD4+ T cell responses in vivo and in vitro
An inverse relationship between T cell receptor affinity and antigen dose during CD4+ T cell responses in vivo and in vitro
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DOI：
10.1073/pnas.96.17.9781
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发表时间：
1999-08-17
影响因子：
11.1
通讯作者：
Kappler, J
中科院分区：
文献类型：
--
作者：
Rees, W;Bender, J;Kappler, J
合成了多聚肽/II类MHC染色试剂，并证明其与T细胞杂交瘤具有适当的特异性，用其中一种试剂在多肽免疫的C57BL/10小鼠体内检测到少量扩大的T细胞群，持续了几个月。这一人群在二次免疫后进一步扩大。将该试剂的结合程度等同于T细胞受体亲和力，我们发现在初始反应的峰值，免疫肽剂量与T细胞受体亲和力几乎没有相关性。然而，在体内或体外，在一次反应或二次刺激几个月后出现的多肽剂量与T细胞的表观受体亲和力之间存在相反的关系。
Multimeric peptide/class II MHC staining reagents were synthesized and shown to bind with appropriate specificity to T cell hybridomas, A small, expanded population of T cells detected with one of these reagents in peptide-immunized C57BL/10 mice persisted for several months. This population expanded further on secondary immunization. Equating the extent of binding of this reagent to T cell receptor affinity, we saw little correlation of immunizing peptide dose to T cell receptor affinity at the peak of the primary response. However, there was an inverse relation between peptide dose and the apparent receptor affinity of the T cells that were present several months after a primary response or after a secondary stimulation either in vivo or in vitro.