Does the geographical gradient of ApoE4 allele exist in China? A systemic comparison among multiple Chinese populations
Does the geographical gradient of ApoE4 allele exist in China? A systemic comparison among multiple Chinese populations
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DOI：
10.1007/s11033-010-0132-0
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发表时间：
2011-01-01
影响因子：
2.8
通讯作者：
Du, Peng Fei
中科院分区：
文献类型：
--
作者：
Hu, Peng;Qin, Yuan Han;Du, Peng Fei
载脂蛋白E（apoE）的等位基因频率在世界各地差异很大。在欧洲，e4频率存在明显的南北梯度，e4携带者的比例从南部的10-15%上升到北部的40-50%。其机制可能与e4携带者不太可能发生维生素D缺乏症有关。此外，亚洲人群的e4频率传统上低于欧洲人，这可能部分归因于最近西方世界的食物供应稀缺或不规则。然而，这些地理分布梯度在中国是否存在还不清楚。采用聚合酶链反应-限制性片段长度多态性（RFLP）分析方法，对南宁市200名儿童的ApoE基因型进行了检测。其他18个群体的等位基因频率数据收集自已发表的来源，并与来自不同地理资源的纬度和经度信息进行相关。在我们的研究对象中，apoE基因型频率分别为E3/E3：73.0%，E3/E2：15.0%，E4/E3：5.0%，E4/E4：5.0%，E4/E2：2.0%，等位基因频率分别为e2：8.5%，e3：83.0%，e4：8.5%。结果表明，中国19个人群共3,679人，等位基因频率分别为e2：7.6%，e3：85.5%，e4：6.9%，e4携带者比例从昆明的4.9%到哈尔滨的17.5%。多人群系统比较发现，e4等位基因频率分布与纬度北相关（r = 0.586，P = 0.008），与经度东相关不显著（r =-0.018，P = 0.942）。结论：中国人群apoE 4等位基因存在南北向的分布梯度，而不存在东西向的分布梯度。
The allelic frequencies of apolipoprotein E (apoE) vary substantially around the world. There is a conspicuous south-to-north gradient of e4 frequencies in Europe, with the proportion of e4 carriers from only 10-15% in the south to 40-50% in the north. The mechanism may be related to the possibility that e4 carriers are less likely to develop vitamin D deficiency. In addition, Asian populations traditionally have lower e4 frequency than Europeans, which may be attributed in part to the scarce or irregular food supplies in Western world in the recent past. However, whether these geographical distribution gradients exist in China is yet unknown. ApoE genotypes of 200 children from Nanning City were determined by PCR-restriction fragment length polymorphism (RFLP) analysis. Allele frequency data of 18 other populations were collected from published sources and correlated with latitude and longitude information from different geographic resources. In our subjects, the frequencies of apoE genotypes were E3/E3: 73.0%, E3/E2: 15.0%, E4/E3: 5.0%, E4/E4: 5.0%, and E4/E2: 2.0%; the frequencies of apoE alleles were e2: 8.5%, e3: 83.0%, and e4: 8.5%, respectively. The total sample consisted of 3,679 individuals from 19 Chinese populations; the allelic frequencies were e2: 7.6%, e3: 85.5%, and e4: 6.9%, respectively; the proportion of e4 carriers was from 4.9% in Kunming to 17.5% in Harbin. Systemic comparison among multiple Chinese populations revealed that positive correlation existed between the e4 allele frequency distribution and latitude north (r = 0.586, P = 0.008), but no correlation of the e4 allele frequency distribution with longitude east was found (r = -0.018, P = 0.942). We conclude that there is a south-to-north, but not an east-to-west gradient for the apoE4 allele in China.