Definition of additional functional ligands for Ly49I(B6) using FVBLy49I(B6) transgenic mice and B6 natural killer cell effectors.
Definition of additional functional ligands for Ly49I(B6) using FVBLy49I(B6) transgenic mice and B6 natural killer cell effectors.
复制标题
使用 FVBLy49I(B6) 转基因小鼠和 B6 自然杀伤细胞效应器定义 Ly49I(B6) 的其他功能配体。
DOI：
10.1097/00007890-200211270-00018
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发表时间：
2002
期刊：
影响因子：
6.2
通讯作者：
Bennett,Michael
中科院分区：
文献类型：
--
作者：
Morris,MargaretA;Koulich,Elena;Liu,Jingxuan;Arora,Veera;George,ThaddeusC;Schatzle,JohnD;Kumar,Vinay;Bennett,Michael
背景：自然杀伤细胞（NK）利用抑制性Ly 49受体与主要组织相容性（MHC）I类分子相互作用，将自身细胞与外来细胞区分开来。抑制性受体可以识别多个MHC I类分子。定义Ly 49受体配体的研究由于受体在NK细胞上以重叠亚群表达的事实而复杂化。结合研究可以预测的MHC I类分子是Ly 49受体的配体，但功能测试需要证实结果从bindingstudy.Methods。我们开发Ly 49受体转基因小鼠和研究Ly 49 I B6在FVB的功能。使用骨髓移植试验测定Ly 49 IB 6转基因小鼠以确定Ly 49 IB 6的另外的功能性配体。我们还使用了荧光激活细胞分选分离特定群体的B6 NK细胞轴承Ly 49 I作为效应器在51铬释放测定对一个面板的刀豆球蛋白A blast targets.Results.Bone骨髓移植研究表明，H2-K B，H2 S，和H2 V作为Ly 49 I B6的功能性配体。体外细胞毒性试验表明，Ly 49 I识别H2 q，但不识别H2 d或H2 k，靶细胞抑制NK killing.Conclusions.These data added support to previous binding studies by showing functional interactions between the B6-strain Ly 49 I and H2-K B，H2 s，H2 v，and H2 q class I antigen.
Background.Natural killer (NK) cells use inhibitory Ly49 receptors to differentiate self from foreign cells based on interactions with major histocompatibility (MHC) class I molecules. Inhibitory receptors may recognize multiple MHC class I molecules. Studies to define ligands for the Ly49 receptors are complicated by the fact that receptors are expressed in overlapping subsets on NK cells. Binding studies can predict which MHC class I molecules are ligands for Ly49 receptors, but functional tests are required to substantiate results from binding studies.Methods.We developed Ly49 receptor transgenic mice and studied the function of Ly49I B6 in FVB. Ly49I B6 transgenic mice using bone marrow transplantation assays to determine additional functional ligands for Ly49I B6. We have also used fluorescence-activated cell sorting to isolate specific populations of B6 NK cells bearing Ly49I for use as effectors in 51 chromium-release assays against a panel of Concanavalin A blast targets.Results.Bone marrow transplantation studies indicate that H2-K b, H2 s, and H2 v serve as functional ligands for Ly49I B6. In vitro cytotoxicity assays indicate that Ly49I recognizes H2 q, but not H2 d or H2 k, target cells to inhibit NK killing.Conclusions.These data add support to previous binding studies by showing functional interactions between the B6-strain Ly49I and H2-K b, H2 s, H2 v, and H2 q class I antigens.