Temporal and spatial expression patterns of biomineralization proteins during early development in the stony coral Pocillopora damicornis
Temporal and spatial expression patterns of biomineralization proteins during early development in the stony coral Pocillopora damicornis
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石珊瑚 Pocillopora damicornis 早期发育过程中生物矿化蛋白的时空表达模式
DOI：
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发表时间：
2016
期刊：
影响因子：
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通讯作者：
P. Falkowski
中科院分区：
文献类型：
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作者：
T. Mass;H. Putnam;Jeana L. Drake;Ehud Zelzion;R. Gates;D. Bhattacharya;P. Falkowski
造礁珊瑚开始是非钙化的幼虫，在沉淀后，迅速开始附着骨骼和富含蛋白质的骨骼有机基质，将它们附着在珊瑚礁上。在这里，我们的特点的时间和空间的表达模式的一套生物矿化基因在三个阶段的幼虫发育的造礁珊瑚Pocillopora damicornis：第一阶段，新发布的第二阶段，oral-aborally压缩和第三阶段，解决和钙化的苗。转录组分析揭示了3882个差异表达基因，这些基因在三个发育阶段聚集成四种明显不同的表达变化模式。免疫定位分析进一步揭示了珊瑚富酸蛋白（CARP）在新兴骨骼的整体架构中的空间排列。这些结果提供了第一次分析的生物矿化的“工具包”在石珊瑚的早期生活史的时间。
Reef-building corals begin as non-calcifying larvae that, upon settling, rapidly begin to accrete skeleton and a protein-rich skeletal organic matrix that attach them to the reef. Here, we characterized the temporal and spatial expression pattern of a suite of biomineralization genes during three stages of larval development in the reef-building coral Pocillopora damicornis: stage I, newly released; stage II, oral-aborally compressed and stage III, settled and calcifying spat. Transcriptome analysis revealed 3882 differentially expressed genes that clustered into four distinctly different patterns of expression change across the three developmental stages. Immunolocalization analysis further reveals the spatial arrangement of coral acid-rich proteins (CARPs) in the overall architecture of the emerging skeleton. These results provide the first analysis of the timing of the biomineralization ‘toolkit’ in the early life history of a stony coral.
影响因子：
3.4
作者：
Treccani, Laura;Mann, Karlheinz;Fritz, Monika
通讯作者：
Fritz, Monika
DOI：
10.1111/j.1365-2818.2003.01263.x
发表时间：
2003-12-01
期刊：
JOURNAL OF MICROSCOPY-OXFORD
影响因子：
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作者：
Blank, S;Arnoldi, M;Fritz, M
通讯作者：
Fritz, M