Sensitive determination of D-amino acids in mammals and the effect of D-amino-acid oxidase activity on their amounts
Sensitive determination of D-amino acids in mammals and the effect of D-amino-acid oxidase activity on their amounts
复制标题
DOI：
10.1248/bpb.28.1578
复制
发表时间：
2005-09-01
影响因子：
2
通讯作者：
Zaitsu, K
中科院分区：
文献类型：
--
作者：
Hamase, K;Konno, R;Zaitsu, K
哺乳动物组织中微量D-氨基酸的测定仍然是分离科学中具有挑战性的主题。在这篇综述中，各种气相色谱和高效液相色谱方法进行了讨论，包括高选择性和灵敏的柱切换程序。在此基础上，阐明了D-天冬氨酸、D-丝氨酸、D-丙氨酸、D-亮氨酸和D-脯氨酸在小鼠脑内的分布。作为哺乳动物体内D-氨基酸含量的调节机制，我们重点研究了D-氨基酸氧化酶催化D-氨基酸的降解。利用缺乏D-氨基酸氧化酶活性的突变小鼠品系，研究了酶活性对各种D-氨基酸的量和分布的影响。
The determination of small amounts of D-amino acids in mammalian tissues is still a challenging theme in the separation sciences. In this review, various gas-chromatographic and high-performance liquid chromatographic methods are discussed including highly selective and sensitive column-switching procedures. Based on these methods, the distributions of D-aspartic acid, D-Serine, D-alanine, D-leucine and D-proline have been clarified in the mouse brain. As the regulation mechanisms of D-amino acid amounts in mammals, we focused on the D-amino-acid oxidase, which catalyzes the degradation Of D-amino acids. Using the mutant mouse strain lacking D-amino-acid oxidase activity, the effects of the enzymatic activity on the amounts and distributions of various D-amino acids have been investigated.