Tangential Flow Microfluidics for the Capture and Release of Nanoparticles and Extracellular Vesicles on Conventional and Ultrathin Membranes
Tangential Flow Microfluidics for the Capture and Release of Nanoparticles and Extracellular Vesicles on Conventional and Ultrathin Membranes
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DOI：
10.1002/admt.201900539
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发表时间：
2019-09-20
影响因子：
6.8
通讯作者：
Gaborski, Thomas
中科院分区：
文献类型：
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作者：
Dehghani, Mehdi;Lucas, Kilean;Gaborski, Thomas
膜已广泛用于生物物种的纯化和分离。一个持续存在的挑战是从浓缩补料溶液中纯化物种，例如从生物液体中纯化细胞外囊泡 (EV)。研究了一种分离微米级和纳米级物质的新方法，称为切向流分析物捕获 (TFAC)，它是传统切向流过滤的延伸。最初，对正常流过滤 (NFF) 和 TFAC 在超薄纳米膜上从血浆中纯化 EV 进行了比较。 NFF 会导致蛋白质饼快速形成，完全掩盖任何捕获的 EV，并阻止进一步跨膜运输。另一方面，TFAC 显示以最小的污染捕获 CD63 阳性小型 EV。探索了使用 TFAC 在膜孔上捕获目标物质，进行清洗，然后在不依赖于亲和力或化学相互作用的物理过程中释放。使用超薄膜和常规厚度膜上的模型颗粒研究了该过程。使用两种膜观察到模型颗粒的成功捕获和释放。超薄纳米膜在显着降低的压力下表现出更高的捕获和释放效率，这表明超薄纳米膜非常适合精细纳米级颗粒（例如 EV）的 TFAC。
Membranes have been used extensively for the purification and separation of biological species. A persistent challenge is the purification of species from concentrated feed solutions such as extracellular vesicles (EVs) from biological fluids. Investigated is a new method to isolate micro- and nanoscale species termed tangential flow for analyte capture (TFAC), which is an extension of traditional tangential flow filtration. Initially, EV purification from plasma on ultrathin nanomembranes is compared between both normal flow filtration (NFF) and TFAC. NFF results in rapid formation of a protein cake which completely obscures any captured EVs and also prevents further transport across the membrane. On the other hand, TFAC shows capture of CD63 positive small EVs with minimal contamination. The use of TFAC to capture target species over membrane pores, wash, and then release in a physical process that does not rely upon affinity or chemical interactions is explored. This process is studied with model particles on both ultrathin and conventional thickness membranes. Successful capture and release of model particles is observed using both membranes. Ultrathin nanomembranes show higher efficiency of capture and release with significantly lower pressures indicating that ultrathin nanomembranes are well-suited for TFAC of delicate nanoscale particles such as EVs.