Standardized broth microdilution antimicrobial susceptibility testing of Francisella tularensis subsp holarctica strains from Europe and rare Francisella species
Standardized broth microdilution antimicrobial susceptibility testing of Francisella tularensis subsp holarctica strains from Europe and rare Francisella species
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DOI：
10.1093/jac/dks238
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发表时间：
2012-10-01
影响因子：
5.2
通讯作者：
Splettstoesser, Wolf D.
中科院分区：
文献类型：
--
作者：
Georgi, Enrico;Schacht, Erik;Splettstoesser, Wolf D.
土拉菌病是一种在欧洲广泛流行的人畜共患病，由土拉弗朗西斯菌土拉菌亚种（Francisella tularensis subsp.）全北极星由于缺乏标准化的CLSI批准的来自欧洲弗朗西斯菌属菌株的抗生素敏感性数据，选择F.土拉热亚种测定了源自德国、奥地利、法国、西班牙和其它欧洲国家的全北极星菌分离物。Francisella的一些罕见种和亚种，如Francisella philomiragia、F.土拉热亚种novicida和F.土拉热亚种使用阳离子调节的MuellerHinton肉汤和2生长补充物获得MIC数据。采用微量肉汤稀释法对庆大霉素、链霉素、环丙沙星、四环素等14种抗生素进行检测，结果91株细菌对氨基糖苷类、喹诺酮类、四环素、氯霉素均敏感。稀有弗朗西斯菌的抗菌药物敏感性与F.土拉热亚种全北极星菌株对于红霉素，我们检测到两个地理上不同的F。土拉热亚种全北极星分离物在西欧。一组是抗性的，另一组是敏感的。两组在德国的一个小区域重叠，本研究按照CLSI标准进行，为欧洲弗朗西斯菌分离株的抗生素敏感性模式提供了可靠的数据。本研究的标准化方法可用于检测临床标本中的可疑菌落，以指导治疗。因此，氨基糖苷类或喹诺酮类抗生素可作为治疗F。hispaniensis，F. philomiragia或F.土拉热亚种免疫功能低下患者的杀线虫感染。
Tularaemia is a widespread zoonosis in Europe caused by Francisella tularensis subsp. holarctica. Because of a lack of standardized CLSI-approved antibiotic susceptibility data from European Francisella strains, the antibiotic susceptibilities of a selection of F. tularensis subsp. holarctica isolates originating from Germany, Austria, France, Spain and other European countries were determined. Rarely isolated species and subspecies of Francisella such as Francisella philomiragia, F. tularensis subsp. novicida and F. tularensis subsp. mediasiatica as well as the type strain of Francisella hispaniensis were included in this study.MIC data were obtained using cation-adjusted MuellerHinton broth with a 2 growth supplement. The broth microdilution testing system comprised 14 antibiotics, including gentamicin, streptomycin, ciprofloxacin and tetracycline.All of the 91 strains tested were susceptible to aminoglycosides, quinolones, tetracycline and chloramphenicol. The antimicrobial susceptibility of rare Francisellae was similar to the antibiotic profile of F. tularensis subsp. holarctica strains. For erythromycin, we detected two geographically distinct groups of F. tularensis subsp. holarctica isolates in western Europe. One group was resistant and the other one was susceptible. Both groups overlapped in a small region in Germany.Being performed in accordance with CLSI criteria, this study provides reliable data on antibiotic susceptibility patterns of European Francisella isolates. The standardized methodology of this study can be used for testing of suspicious colonies from clinical specimens for therapeutic guidance. Based on the results, aminoglycosides or quinolones are recommended as first-choice antibiotics for the therapy of F. hispaniensis, F. philomiragia or F. tularensis subsp. novicida infections in immunocompromised patients.