Comparative evaluation of HET-CAM and ICE methods for objective assessment of ocular irritation caused by selected pesticide products
Comparative evaluation of HET-CAM and ICE methods for objective assessment of ocular irritation caused by selected pesticide products
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DOI：
10.1016/j.tiv.2021.105150
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发表时间：
2021-03-31
影响因子：
3.2
通讯作者：
Lehel, Jozsef
中科院分区：
文献类型：
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作者：
Budai, Peter;Kormos, Eva;Lehel, Jozsef
用HET-CAM法和ICE法对杀菌剂、除草剂、杀虫剂的眼刺激性进行了比较。基于测试的结果，使用个别方法的分类之间的协议的统计分析是由古德曼-克鲁斯卡尔？的秩相关和科恩？s kappa系数。分类之间一致性的统计分析显示体内和HET-CAM测定结果之间存在显著相关性（76%）。体内试验结果与ICE法结果无显著相关性（64%）。在体外HET-CAM和ICE测试的数据之间发现最弱的相关性。两个体外数据之间的一致性百分比为48%。它们可以被推荐作为一系列试验的一部分，以减少对哺乳动物的实验，并限制或消除对实验动物造成的疼痛和伤害。HET-CAM试验是研究体内潜在结膜刺激的有用工具，而ICE试验可用于详细研究角膜刺激作用。
Eye irritation potency of pesticides (fungicides, herbicides, insecticides) was comparatively tested by HET-CAM and ICE method. Based on the results of the tests the statistical analysis of agreement between classification using individual methods was done by Goodman-Kruskal?s rank correlation and determination (calculation) of Cohen?s kappa coefficient. Statistical analysis of agreement between classification revealed significant correlation between results of in vivo and in HET-CAM assays (76%). There was no significant correlation between result of in vivo and in ICE methods (64%). Weakest correlation was found between the data from in vitro HET-CAM and ICE tests. The percentage of agreement between two in vitro data was 48%. They may be recommended as a part of a battery of tests to reduce experimentation on mammals and to limit or eliminate pain and injury inflicted on experimental animals. The HET-CAM test is a useful tool for studying in vivo the potential conjunctival irritation, while the ICE test can be used to study corneal irritant effects in detail.