CDPK gene expression in salt tolerant rolB and rolC transformed cell cultures of Panax ginseng
CDPK gene expression in salt tolerant rolB and rolC transformed cell cultures of Panax ginseng
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DOI：
10.1007/s10535-010-0112-1
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发表时间：
2010-12-01
影响因子：
1.5
通讯作者：
Zhuravlev, Y. N.
中科院分区：
文献类型：
--
作者：
Kiselev, K. V.;Grishchenko, O. V.;Zhuravlev, Y. N.
CDPK（钙依赖性蛋白激酶）对于植物防御反应的激活非常重要。在这项研究中，我们的目的是寻找人参中 CDPK 表达与耐盐性增强之间的联系。用 W7（CDPK 蛋白抑制剂）处理人参细胞培养物表明 CDPK 蛋白是耐盐所必需的。与对照细胞相比，用 60 mM NaCl 处理的细胞中 PgCDPK1c、PgCDPK2c 和 PgCDPK4a 的表达显着增加，而 PgCDPK1b 和 PgCDPK3a 的表达降低。在 NaCl 处理的细胞中，还出现了新的 CDPK 转录本（PgCDPK3c、PgCDPK4as）。我们还使用了 rolC 和 rolB 转化培养物，rol 基因对 CDPK 表达的影响与盐胁迫的影响相似：除了出现“短”CDPK 转录本外，它们还导致 PgCDPK1c、PgCDPK2c 和 PgCDPK4a 表达显着增加，PgCDPK3a 表达减少。
CDPKs (calcium-depended protein kinases) are of great importance for the activation of defense reactions in plants. In this study, we aimed to find a connection between CDPK expression and increased salt tolerance in Panax ginseng. Treatment of P. ginseng cell cultures with W7 (CDPK protein inhibitor) showed that CDPK proteins were necessary for salt tolerance. Expression of PgCDPK1c, PgCDPK2c and PgCDPK4a was significantly increased in the cells treated with 60 mM NaCl compared to control cells, whereas expression of PgCDPK1b and PgCDPK3a was decreased. In the NaCl-treated cells, new CDPK transcripts also appeared (PgCDPK3c, PgCDPK4as). We also used rolC and rolB transformed cultures and the effects of the rol genes on CDPK expression were similar to the effects of salt stress: they caused a significant increase in the expression of PgCDPK1c, PgCDPK2c, and PgCDPK4a and decreased expression of PgCDPK3a, in addition to the appearance of the "short" CDPK transcripts.