Detection and quantification of Fusarium culmorum and Fusarium graminearum in cereals using PCR assays
Detection and quantification of Fusarium culmorum and Fusarium graminearum in cereals using PCR assays
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DOI：
10.1006/pmpp.1998.0170
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发表时间：
1998-07-01
影响因子：
2.7
通讯作者：
Joyce, D
中科院分区：
文献类型：
--
作者：
Nicholson, P;Simpson, DR;Joyce, D
随机扩增多态性DNA分析被用来确定扩增产物的特征，无论是镰刀菌culmorum或禾谷镰刀菌。对选定的片段进行克隆、测序，并开发允许特异性检测的引物对。culmorum或F. graminearum的PCR产物。建立了F. culmorum和F. graminearum，采用竞争性PCR。食品药品管理采用茎基特异性竞争PCR技术，研究了接种量和接种时间对冬小麦茎基病的影响。高潮真菌定殖的程度，真菌DNA含量测定，在本赛季初接种的植物上更大，并随着分生孢子负荷的增加而增加。食品药品管理利用禾谷镰刀菌特异性竞争PCR技术，研究了产和不产青霉烯的F.禾谷早熟禾结果表明，产青霉烯菌株在小麦上的定殖率高于不产青霉烯菌株，这支持了青霉烯在小麦定殖过程中起毒力因子作用的观点。禾谷早熟禾在这两种情况下，竞争性PCR检测的结果与视觉疾病评估的结果进行了比较。(C)北京：科学出版社.
Random amplified polymorphic DNA assays were used to identify amplification products characteristic of either Fusarium culmorum or Fusarium graminearum. Selected fragments were cloned, sequenced and primer pairs were developed which permitted specific detection off. culmorum or F. graminearum using conventional PCR. Quantitative assays were developed for both F. culmorum and F. graminearum, using competitive PCR. The F. culmorum-specific competitive PCR assay was used to study the effect of inoculum load and timing on stem base disease of winter wheat caused by F. culmorum. The extent of fungal colonization, as measured by fungal DNA content, was greater on plants inoculated earlier in the season and increased with increasing conidial load. The F. graminearum-specific competitive PCR assay was used to study the colonization of wheat grain by trichothecene producing and non-producing isolates of F. graminearum. Colonization of grain by trichothecene producing isolates was greater than that by non-producing isolates, supporting the view that trichothecenes act as virulence factors in the colonization of wheat by F. graminearum. The results from competitive PCR assays were compared with those for visual disease assessment in both instances. (C) 1998 Academic Press.