A potent anti-coagulant RNA aptamer inhibits blood coagulation by specifically blocking the extrinsic clotting pathway
A potent anti-coagulant RNA aptamer inhibits blood coagulation by specifically blocking the extrinsic clotting pathway
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DOI：
10.1160/th06-01-0047
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发表时间：
2006-05-01
影响因子：
6.7
通讯作者：
Kumar, Penmetcha K. R.
中科院分区：
文献类型：
--
作者：
Gopinath, Subash C. B.;Shikamoto, Yasuo;Kumar, Penmetcha K. R.
据报道，一种有效的解毒剂控制的适体作为抗凝剂，通过使用现有的抗凝剂克服了急性出血的并发症。在本研究中，我们提供的证据表明适体与因子 IX 和 IXa 特异性结合并抑制其功能。此外，我们证明适体通过干扰外在途径、阻断因子VIIa和组织因子与因子IX的相互作用的复合物来抑制血液凝固。之前和当前研究的结果表明，适体可能结合在因子IX的EGF1和EGF2结构域附近。
A potent antidote-controlled aptamer, as an anticoagulant, has reportedly overcome the complications of acute bleeding by the administration of available anticoagulants. In the present study, we provide evidence that the aptamer binds specifically to factors IX and IXa and inhibits their functions. Furthermore,we demonstrated that the aptamer inhibits blood coagulation by interfering with the extrinsic pathway, blocking the complex of factor VIIa and tissue factor interactions with factor IX The results from the previous and present studies suggest that the aptamer probably binds in the vicinity of the EGF1 and EGF2 domains of factor IX.