Skate-skin mucin, rich in sulfated sugars and threonine, promotes proliferation of <i>Akkermansia muciniphila</i> in feeding tests in rats and in vitro fermentation using human feces
Skate-skin mucin, rich in sulfated sugars and threonine, promotes proliferation of <i>Akkermansia muciniphila</i> in feeding tests in rats and in vitro fermentation using human feces
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富含硫酸化糖和苏氨酸的滑冰皮粘蛋白在大鼠喂养试验和使用人类粪便的体外发酵中促进 <i>Akkermansia muciniphila</i> 的增殖
DOI：
10.1093/bbb/zbac003
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发表时间：
2022
期刊：
影响因子：
--
通讯作者：
Morita Tatsuya
中科院分区：
文献类型：
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作者：
Miyata Takaaki;Mizushima Takayasu;Miyamoto Nobuyuki;Yamada Takahiro;Hase Koji;Fukushima Michihiro;Nishimura Naomichi;Hino Shingo;Morita Tatsuya
饮食因素影响结肠中嗜粘蛋白阿克曼氏菌（Akkermansia muciniphila，AM）的丰度，受到AM丰度和代谢紊乱之间的负相关性的驱动，引起了人们的关注。我们制备了滑板皮肤粘蛋白（SM）、猪胃粘蛋白（PM）和大鼠胃肠粘蛋白（RM）。SM比PM或RM含有更多的硫酸化糖和苏氨酸。以5种不同苏氨酸含量的SM、PM、RM（15 g/kg）或SM（12 g/kg）为饲料，饲喂对照饲料14 d。SM喂养大鼠盲肠细菌总数和AM数量分别比其他大鼠少和多，但SM不影响微生物物种丰富度。低苏氨酸SM不诱导AM增殖。人粪体外发酵试验表明，SM比PM对AM的增菌率高。总的来说，重度SM硫酸化有利于优先供应SM衍生的氨基糖和苏氨酸，从而促进大鼠和人类粪便中的AM增殖。
Dietary factors, affectAkkermansia muciniphila(AM) abundance in the colon, have attracted attention, driven by the inverse correlation betweenAMabundance and metabolic disorders. We prepared skate-skin mucin (SM), porcine stomach mucin (PM), and rat gastrointestinal mucin (RM). SM contained more sulfated sugars and threonine than PM or RM. Rats were fed a control diet or diets including SM, PM, or RM (15 g/kg), or SM (12 g/kg) from 5 different threonine contents for 14 d. Cecal total bacteria andAMwere less and more numerous, respectively, in SM-fed rats than the others, but SM did not affect microbial species richness. Low-threonine SM did not induceAMproliferation. Thein vitrofermentation with human feces showed that the rate ofAMincrease was greater with SM than PM. Collectively, heavy SM sulfation facilitates a priority supply of SM-derived amino sugars and threonine that promotesAMproliferation in rats and human feces.