Dye dilution proliferation assay: application of the DDPA to identify tumor-specific T cell precursor frequencies in clinical trials.
Dye dilution proliferation assay: application of the DDPA to identify tumor-specific T cell precursor frequencies in clinical trials.
复制标题
染料稀释增殖测定：应用 DDPA 来识别临床试验中肿瘤特异性 T 细胞前体频率。
DOI：
10.1080/08820130701674760
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发表时间：
2007
影响因子：
2.8
通讯作者：
Ernstoff,MarcS
中科院分区：
文献类型：
--
作者：
Schwaab,Thomas;Fisher,JanL;Meehan,KennethR;Fadul,CamiloE;Givan,AliceL;Ernstoff,MarcS
对免疫效应子和调节途径的更好理解导致了创新和复杂的免疫治疗策略。CD8+细胞溶解性T淋巴细胞（CTL）提供了一种常见的肿瘤细胞破坏途径。外周血CTL区室通常包含少数抗肿瘤CD8+淋巴细胞，并且在临床试验期间确定它们的数量是各种实验室方法的焦点。我们使用染料稀释增殖试验（DDPA）监测了外周血中肿瘤特异性CD8+和CD4+淋巴细胞前体细胞频率。我们总结了DDPA在多参数抗原特异性检测中的应用经验，详细介绍了它的一些复杂性和优势。我们提供了我们的临床试验结果的例子，显示了在新型免疫治疗试验中接受治疗的患者的肿瘤特异性CD8+和CD4+前体频率（PF）数据。
A better understanding of immune effector and regulatory pathways has led to innovative, and complex, immunotherapy strategies. CD8+cytolytic T lymphocytes (CTL) provide one common pathway of tumor cell destruction. The peripheral blood CTL compartment typically comprises a minority of anti-tumor CD8+lymphocytes and the determination of their number during clinical trials is the focus of various laboratory methods. We have monitored tumor specific CD8+as well as CD4+lymphocyte precursor frequencies in the peripheral blood using a Dye Dilution Proliferation Assay (DDPA). We summarize our experience applying DDPA in a multi-parameter, antigen-specific assay, detailing some of its complexities and advantages. We provide examples of our clinical trial results showing tumor-specific CD8+and CD4+precursor frequency (PF) data in patients being treated on novel immunotherapy trials.