Effects of the ecological factors on hydrogen production and [Fe–Fe]-hydrogenase activity in Ethanoligenens harbinense YUAN-3
Effects of the ecological factors on hydrogen production and [Fe–Fe]-hydrogenase activity in Ethanoligenens harbinense YUAN-3
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DOI：
10.1016/j.ijhydene.2015.02.015
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发表时间：
2015-06
影响因子：
7.2
通讯作者：
Luyan Zhang;J. Chung;N. Ren;R. Sun
中科院分区：
文献类型：
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作者：
Luyan Zhang;J. Chung;N. Ren;R. Sun
氢化酶是生物产氢代谢中重要的限速酶之一。氢酶的活性影响着产氢的速度。以一株新型高效产氢细菌Ethanoligenens harbinenseYUAN-3为研究对象，研究了产氢酶活性与产氢性能的关系。研究了温度、pH、C/N比、Mg ~（2+）、Fe ~（2+）浓度等生态因子对产氢和氢酶活性的影响。沿着发酵过程，氢化酶的活性在达到最大速率2.18 molH 2 molGlucose −1（H2产量为1942.5 mL L−1）后迅速下降。菌株E的氢酶活力最高。harbinenseYuan-3的最适培养条件为35 °C，pH 4.5，最适C/N为22.4。同时发现300 mg L− 1 MgCl 2·6 H2O和300 mg L− 1FeSO 4·7 H2O的最佳浓度对产氢起关键作用。最高产氢量提高到2.62 molH 2 molGlucose −1（H2产率为2138.5 mL L−1），氢化酶的最大活性为0.352 U mg− 1蛋白。
Hydrogenase is one of the vital rate-limiting enzymes in biohydrogen production metabolism. The activity of hydrogenase impacts the speed of hydrogen production. In the present study, the novel high efficient H2-producing bacteriumEthanoligenens harbinenseYUAN-3 was selected for characterization of the relationship between hydrogenase activity and its hydrogen production performance. The effects of the ecological factors of temperature, pH, C/N ratio Mg2+, and Fe2+concentration on hydrogen production and the activity of hydrogenase were investigated. The activity of hydrogenase decreased quickly after reaching a maximum rate of 2.18 molH2molGlucose−1(H2yield of 1942.5 mL L−1) along with the fermentation process. The highest hydrogenase activity of strainE. harbinenseYUAN-3 was optimized under conditions of 35 °C, pH 4.5, and the optimal C/N ratio of 22.4. It was also found that the optimal concentration of 300 mg L−1MgCl2·6H2O and 300 mg L−1FeSO4·7H2O were playing key roles in hydrogen production. The highest hydrogen production was improved to 2.62 molH2molGlucose−1(H2yield of 2138.5 mL L−1) with the maximum activity of the hydrogenase 0.352 U mg−1protein.