AFLP-based genetic relationships among maize inbred lines selected in a climatically temperate location.
AFLP-based genetic relationships among maize inbred lines selected in a climatically temperate location.
复制标题
在气候温和地区选择的玉米自交系之间基于 AFLP 的遗传关系。
作者：
A. Chittó;M. Bertolini;H. Hartings;A. Verderio;M. Motto
对一系列71个意大利自交系进行AFLP分析，认为这些自交系代表了保存在Istituto Sperimentale per la Cerealicoltura的贝加莫玉米站的育种材料。20个美国参考系，独特的主要杂种优势群体提供了遗传多样性的基础上，意大利自交系相关的评估其相对遗传关系。共鉴定出682个多态性片段，用于计算成对遗传距离，并随后推导出系统聚类。自交系的聚类反映了系谱信息，并导致鉴定了来自兰开斯特可靠作物（LSC）和爱荷华州硬股票合成（BSSS）杂种优势育种材料的主要群体。AMOVA统计，对建立的遗传结构，揭示了杂种优势组内的方差的比例很高，表明在育种计划的变化一直保持在自交系宪法的群体内。AMOVA结果证实了多样性分析，揭示了高水平的遗传变异内和杂种优势群之间。总之。AFLP显示出高度的信息量，并构成了一个有价值的工具，分配自交系，并测定遗传变异，杂种优势群。
AFLP analysis was performed on a series of 71 Italian inbred lines, considered representative of the breeding material maintained at the Bergamo Maize Station of the Istituto Sperimentale per la Cerealicoltura. Twenty American reference lines, distinctive for the major heterotic groups available supplied the basis of genetic diversity to which the Italian inbreds were correlated in the assessment of their relative genetic relationships. A total of 682 polymorphic fragments were identified, which were used to calculate pairwise genetic distances and to subsequently derive hierarchical clustering. Clustering of the inbreds reflected pedigree information and resulted in the identification of major groups derived from Lancaster Sure Crop (LSC) and Iowa Stiff Stock Synthetic (BSSS) heterotic breeding material. AMOVA statistics, performed on the established genetic structure, revealed a high proportion of variance within heterotic groups, indicating that in the breeding program variability had been maintained within the groups during inbred line constitution. AMOVA results were corroborated by diversity analysis, which revealed a high level of genetic variability within and across heterotic groups. In conclusion. AFLPs appeared highly informative and constituted a valuable tool for assigning inbred lines to, and assaying genetic variability across, heterotic groups.