The ATE genes are responsible for repression of trans differentiation into xylem cells in Arabidopsis
The ATE genes are responsible for repression of trans differentiation into xylem cells in Arabidopsis
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DOI：
10.1104/pp.104.055145
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发表时间：
2005-01-01
期刊：
影响因子：
7.4
通讯作者：
Fukuda, H
中科院分区：
文献类型：
--
作者：
Sawa, S;Demura, T;Fukuda, H
我们分离了拟南芥 (Arabidopsis thaliana) 的三个隐性突变体，它们显示木质部特异性标记 pAtxyn3::YFP 的异位表达。遗传分析表明，这些表型是由三个不同基因的突变引起的，这些基因被称为异常气管元件形成相关基因表达（ate1-3）。 ate1突变体表现出正常的DR5::GUS基因表达模式，ate1突变不影响van3和pin1突变体中异常血管模式的形成，表明ate1突变不影响生长素调控的血管模式组织。 ate突变体表现出异位木质素沉积、图案化次生壁增厚和细胞死亡，这是异位表达pAtxyn3::YFP基因的细胞中成熟气管元件(TE)的特征。在切除下胚轴的 TE 诱导系统中，ate 突变体的实质组织中快速诱导异位 TE 形成。此外，逆转录聚合酶链式反应实验表明，TE 形成相关基因的表达在 ate 突变体中上调。 ate1 突变还导致另一个木质部特异性标记基因 pAt3g62160::YFP 的异位表达。总体而言，我们的结果表明，ATE 基因负责拟南芥中转分化为 TE 的原位抑制，并且可能是转分化掩蔽系统的参与者。
We isolated three recessive mutants of Arabidopsis (Arabidopsis thaliana) showing ectopic expression of the xylem-specific marker, pAtxyn3::YFP. Genetic analysis indicated that the phenotypes were caused by mutations in three different genes, designated Abnormal Tracheary Element formation-related gene expression (ate1-3). The ate1 mutants showed a normal DR5::GUS gene expression pattern, and the ate1 mutation did not affect the abnormal vascular pattern formation in the van3 and pin1 mutants, indicating that the ate1 mutation does not affect the vascular pattern organization governed by auxin. The ate mutants showed ectopic lignin deposition, patterned secondary wall thickenings, and cell death, which are characteristic of mature tracheary elements (TEs) in cells ectopically expressing the pAtxyn3::YFP gene. Ectopic TE formation was rapidly induced in parenchymal tissue of the ate mutants in a TE-inducible system with excised hypocotyl. Furthermore, reverse transcription-polymerase chain reaction experiments showed that the expression of TE formation-related genes is up-regulated in the ate mutants. The ate1 mutation also caused ectopic expression of another xylem-specific marker gene, pAt3g62160::YFP. Overall, our results suggest that the ATE genes are responsible for the in situ repression of transdifferentiation into TEs in Arabidopsis and Could be participants in the transdifferentiation-masking system.