MEDIAL EDGE EPITHELIUM FATE TRACED BY CELL LINEAGE ANALYSIS DURING EPITHELIAL MESENCHYMAL TRANSFORMATION INVIVO
MEDIAL EDGE EPITHELIUM FATE TRACED BY CELL LINEAGE ANALYSIS DURING EPITHELIAL MESENCHYMAL TRANSFORMATION INVIVO
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DOI：
10.1016/0012-1606(92)90071-n
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发表时间：
1992-12-01
影响因子：
2.7
通讯作者：
SANK, AC
中科院分区：
文献类型：
--
作者：
SHULER, CF;HALPERN, DE;SANK, AC
活体细胞标记技术用于追踪体内腭融合过程中内侧边缘上皮（MEE）细胞的命运。小鼠腭组织在子宫内用 DiI 标记。胎儿在子宫内继续发育，并在腭个体发育的几个后期阶段检查了次级腭组织。 DiI 的存在和分布与细胞表型特异性标记物的存在相关。在腭融合的初始阶段，DiI 标记的 MEE 出现在中线位置。这些细胞附着在完整的含有层粘连蛋白的基底膜上，并含有角蛋白中间丝。在腭发育的后期阶段，DiI 标记的 MEE 没有通过完整的基底膜与间充质分离，并且不含角蛋白。在胎儿发育晚期，间充质中存在没有上皮形态的 DiI 标记细胞。 DiI标记的细胞从上皮表型到间充质表型的转变与上皮-间质转化的命运一致，而不是程序性细胞死亡。
Vital cell labeling techniques were used to trace the fate of the medial edge epithelial (MEE) cells during palatal fusionin vivo. Mouse palatal tissues were labeledin uterowith DiI. The fetuses continued to developin uteroand tissues of the secondary palate were examined at several later stages of palatal ontogeny. The presence and distribution of DiI was correlated with the presence of cell phenotype-specific markers. During the initial stages of palatal fusion the DiI-labeled MEE were present in the midline position. These cells were attached to an intact laminin-containing basement membrane and contained keratin intermediate filaments. At later stages of palatogenesis the DiI-labeled MEE were not separated from the mesenchyme by an intact basement membrane and did not contain keratin. In late fetal development, DiI-labeled cells without an epithelial morphology were present in the mesenchyme. The transition of the DiI-labeled cells from an epithelial phenotype to a mesenchymal phenotype is consistent with a fate of epithelial-mesenchymal transformation rather than programmed cell death.