Growth and energy status of arrowhead tubers, pondweed turions and rice seedlings under anoxic conditions
Growth and energy status of arrowhead tubers, pondweed turions and rice seedlings under anoxic conditions
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DOI：
10.1046/j.1365-3040.1999.00439.x
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发表时间：
1999-05-01
影响因子：
7.3
通讯作者：
Kuramochi, H
中科院分区：
文献类型：
--
作者：
Ishizawa, K;Murakami, S;Kuramochi, H
研究了慈姑（Sagittaria pygmaea Mig.）和眼子菜（Potamogeton distinctus A.）Benn），与水稻（Oryza sativa L.）慈姑块茎的芽伸长在1千帕O-2左右增强，而在空气中的眼子菜turions是轻微的，并达到最大值，在没有O-2。乙醇脱氢酶（ADH）活性的厌氧增强发生在水稻胚芽鞘，而不是慈姑和眼子菜芽。慈姑和眼子菜的芽在厌氧条件下比水稻胚芽鞘保持更稳定的能量状态。慈姑和眼子菜的总腺苷酸含量在厌氧条件下保持不变。腺苷酸能荷在两个芽中至少保持8d在0.8以上的高而稳定的水平。用淀粉凝胶电泳法分离慈姑茎中ADH的三种形式，表明在好氧和厌氧条件下，每种形式的ADH活性不同。将S-35标记的Cys和Met掺入慈姑芽中的可溶性蛋白质中，表明蛋白质生物合成活跃，并参与了一组特殊的多肽在厌氧生活中。
The responses of two aquatic plants, arrowhead (Sagittaria pygmaea Mig.) and pondweed (Potamogeton distinctus A. Benn), to anoxia were compared with those of rice (Oryza sativa L.), Shoot elongation of arrowhead tubers was enhanced at around 1 kPa O-2, whereas that of pondweed turions was slight in air and reached a maximum in the absence of O-2. Anaerobic enhancement of alcohol dehydrogenase (ADH) activity took place in rice coleoptiles but not in arrowhead and pondweed shoots. Shoots of both arrowhead and pondweed maintained a more stable energy status than did the rice coleoptile under anaerobic conditions. Total adenylate nucleotide contents of arrowhead and pondweed shoots were constant under anaerobic conditions. Adenylate energy charge in both shoots remained at a high and stable level of more than 0.8 for at least 8 d. Three forms of ADH from arrowhead shoots were separated by starch gel electrophoresis, showing that the activity of each ADH form was different under aerobic and anaerobic conditions. The incorporation of S-35-labelled Cys and Met into soluble proteins in arrowhead shoots showed active protein biosynthesis and an involvement of a special set of polypeptides in the anaerobiosis.