ORGANOGENESIS IN CARNATION
ORGANOGENESIS IN CARNATION
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DOI：
10.21273/jashs.116.6.1108
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发表时间：
1991-11-01
影响因子：
1.9
通讯作者：
JANICK, J
中科院分区：
文献类型：
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作者：
FREY, L;JANICK, J
香石竹不定芽再生的研究受基因型、外植体来源和植物生长调节剂平衡的影响。从花瓣、花萼、节、节间和叶再生植株，但从所有三个被检查的栽培品种（“Scania”、“改良白色Sim”、“Sandra”）中仅花瓣、花萼和节再生。花瓣在补充有0.05 μ M TDZ和0.5 μ M NAA的Murashige和Skoog培养基上实现了最大增殖。不定芽起源于维管区附近的细胞，也可能来自花瓣的表皮细胞和来自其他外植体的器官发生的愈伤组织。没有证据表明嵌合体分离花瓣或愈伤组织，但体细胞克隆变异（3.3%）观察到花瓣色调和植物矮化。不稳定的颜色模式中观察到组织培养的再生体的“斯堪的纳维亚和”改进白色西姆“的类型和频率类似的繁殖体来自扦插;没有观察到组织培养或扦插衍生植物的”桑德拉“。使用的化学名称：N-苯基-N '-1，2，3-噻二唑-5-基脲[噻苯隆（TDZ）]; 1-萘乙酸（NAA）。
Shoot regeneration in carnation (Dianthus caryophyllus L.) was influenced by genotype, explant source, and plant growth regulator balance. Plants were regenerated from petals, calyxes, nodes, internodes, and leaves, but only petals, calyxes, and nodes were regenerative from all three cultivars examined ('Scania', 'Improved White Sim', 'Sandra'). Maximum proliferation was achieved with petals on Murashige and Skoog medium supplemented with 0.05-mu-M TDZ and 0.5-mu-M NAA. Shoot initiation originated from cells near vascular regions and perhaps from epidermal cells in petals and via organogenic callus from other explants. There was no evidence of chimeral separation from petals or callus, but somaclonal variants (3.3%) were observed involving petal hue and plant dwarfness. Unstable color patterns were observed in tissue-cultured regenerants of 'Scanial and 'Improved White Sim' similar in type and frequency to propagules derived from cuttings; none were observed for tissue-cultured or cutting-derived plants of 'Sandra'. Chemical names used: N-phenyl-N'-1,2,3-thiadiazol-5-ylurea [thidiazuron (TDZ)]; 1-napthaleneacetic acid (NAA).