Overexpression of HSP70 is induced by ionizing radiation in C3H 10T1/2 cells and protects from DNA damage
Overexpression of HSP70 is induced by ionizing radiation in C3H 10T1/2 cells and protects from DNA damage
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DOI：
10.1016/s0887-2333(03)00116-4
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发表时间：
2003-10-01
影响因子：
3.2
通讯作者：
Camatini, M
中科院分区：
文献类型：
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作者：
Calini, V;Urani, C;Camatini, M
热、紫外线、γ-射线和化学物质等多种应激可导致DNA损伤，诱导热休克蛋白（Hsps），特别是Hsp 70。Hsps的细胞保护功能尚未完全了解，虽然这些蛋白质作为分子伴侣或细胞内活性氧（ROS）水平的调节剂。最近，热休克蛋白已被提出在紫外线或γ射线照射后的DNA修复中发挥重要作用。电离辐射通过直接相互作用或通过产生自由基和ROS靶向DNA分子。当暴露于γ射线时，C3 H 10 T1/2细胞是辐射敏感的，因此我们决定使用它们来研究电离辐射后Hsp的诱导及其对DNA损伤的保护作用。在这里，我们展示了热休克蛋白的诱导γ射线，并探讨在不同剂量照射后的表达动力学。我们还表明，热休克蛋白70过表达作为一种辐射防护机制对第一次事件的DNA损伤，并增加长期生存能力。对细胞周期的初步研究没有证据表明诱导型Hsp 70对其有显著的保护作用。（C）2003 Elsevier Ltd.保留所有权利。
Various kinds of stress such as heat, UV, gamma-rays and chemicals that cause DNA damage induce heat shock proteins (Hsps), and in particular Hsp70. The Hsps cytoprotective function is not fully understood, although these proteins act as molecular chaperones or modulators of intracellular levels of reactive oxygen species (ROS). Recently, Hsps have been proposed to play a significant role in DNA repair after UV or gamma-ray irradiation. Ionizing radiation targets DNA molecules either via direct interaction or via production of free radicals and ROS. When exposed to gamma-rays C3H 10T1/2 cells are radiosensitive, therefore we decided to use them to investigate Hsp induction after ionizing radiation and their protective role against DNA damage. Here we demonstrate the induction of Hsps by gamma-rays, and investigate the kinetics of expression after irradiation at different doses. We also show that Hsp70 overexpression acts as a radioprotective mechanism towards the first event of DNA damage and increases long term viability. A preliminary investigation on the cell cycle does not evidence a significant protective action of inducible Hsp70 on it. (C) 2003 Elsevier Ltd. All rights reserved.