The anaemia of chronic renal failure in sheep: studies in vitro.
The anaemia of chronic renal failure in sheep: studies in vitro.
复制标题
绵羊慢性肾衰竭贫血：体外研究。
DOI：
10.1111/j.1365-2141.1984.tb03996.x
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发表时间：
1984
影响因子：
6.5
通讯作者：
Adamson,JW
中科院分区：
文献类型：
--
作者：
Mladenovic,J;Eschbach,JW;Garcia,JF;Adamson,JW
综述：在尿毒症期间蓄积的抑制物的存在被认为是慢性肾功能衰竭（CRF）患者发生贫血的一个重要因素。为了确定尿毒症血清中的因素是否抑制红细胞生成，在手术诱导CRF之前和之后从绵羊中获得样品。体外检测血清对红系细胞集落生长的影响。在最佳促红细胞生成素（Ep）剂量下，在血清型匹配或自体绵羊骨髓细胞培养物中用绵羊血清替代胎牛血清（30%浓度）。针对正常骨髓（22）和尿毒症骨髓（20）检测了来自5只动物的42对血清。在7/42个随机对中，与来自同一动物的正常样本相比，在存在尿毒症血清的情况下，红系细胞集落生长减少20%。在其余的培养物中，尿毒症血清刺激或支持红细胞集落生长以及正常血清。当单独分析结果时，5只动物中只有1只动物的血清显示出对红系集落生长的体外抑制最小（10%）。本研究采用相容性靶细胞进行前瞻性研究，对尿毒症毒素显著抑制体外红细胞生成的假说提出质疑。这些数据与Ep在该绵羊模型中的体内反应有关，提示Ep对CRF贫血有治疗作用。
Summary.The presence of inhibitors which accumulate during uraemia has been postulated as a significant factor in the development of anaemia in chronic renal failure (CRF). To determine whether factors in uraemic serum depress erythropoiesis, samples were obtained from sheep prior to and after surgical induction of CRF. The sera were tested invitrofor their effect on erythroid colony growth. The sheep sera were substituted for fetal calf serum (30% concentration) in cultures of serotype‐matched or autologous sheep marrow cells at optimal doses of erythropoietin (Ep). Forty‐two paired sera from five animals were tested against normal (22) and uraemic marrow (20). In 7/42 random pairs, erythroid colony growth was decreased by 20% in the presence of uraemic serum when compared to a normal sample from the same animal. In the remainder of the cultures, uraemic sera stimulated or supported erythroid colony growth as well as normal sera. When the results were analysed individually, serum from only one of five animals showed minimal (10%)in vitroinhibition of erythroid colony growth. This study, performed in a prospective manner utilizing compatible target cells, disputes the hypothesis that uraemic toxins significantly inhibitin vitroerythropoiesis. These data correlate with thein vivoresponse to Ep in this sheep model, and suggest Ep would be effective in treating the anaemia of CRF.