A maturational model for the study of airway smooth muscle adaptation to mechanical oscillation.
A maturational model for the study of airway smooth muscle adaptation to mechanical oscillation.
复制标题
用于研究气道平滑肌对机械振荡的适应的成熟模型。
DOI：
10.1139/y05-057
复制
发表时间：
2005
期刊：
影响因子：
--
通讯作者：
Murphy,ThomasM
中科院分区：
文献类型：
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作者：
Wang,Lu;Chitano,Pasquale;Murphy,ThomasM
研究表明，深吸气对气道平滑肌 (ASM) 施加的机械拉伸会降低 ASM 随后的收缩反应。这种肌肉拉长和收缩的被动动作对于正常受试者对抗支气管痉挛是有益的。然而，它对高反应性气道有害，因为它会引发进一步的支气管收缩。尽管正常气道和高反应性气道产生这种相反反应的确切机制尚不清楚，但有人认为这种现象与 ASM 对机械振荡的适应性变化有关。人类和动物的健康未成熟气道均表现出高反应性，但高反应性气道的适应特性是否与正常气道不同仍不清楚。在本文中，我们回顾了 ASM 对机械振荡的适应现象及其与气道高反应性的相关性和含义。我们使用我们实验室开发的成熟动物模型证明了 ASM 适应的年龄特异性表达是突出的。我们对未成熟 ASM 的数据显示，与振荡前产生的最大力相比，长度振荡后不久的收缩力增强。讨论了几种潜在的机制，例如肌源性反应、肌动蛋白聚合的变化或细胞骨架调节蛋白凝集素和波形蛋白数量的变化，这些可能是这种年龄特异性力增强的基础。我们提出了与力传递相关的平滑肌结构的工作模型，这可能有助于阐明负责平滑肌适应的年龄特异性表达的机制。研究 ASM 适应的成熟特征非常重要，因为它可能导致青少年高反应性。关键词：ASM 适应，成熟，支气管保护，气道高反应性。
It has been shown that mechanical stretches imposed on airway smooth muscle (ASM) by deep inspiration reduce the subsequent contractile response of the ASM. This passive maneuver of lengthening and retraction of the muscle is beneficial in normal subjects to counteract bronchospasm. However, it is detrimental to hyperresponsive airways because it triggers further bronchoconstriction. Although the exact mechanisms for this contrary response by normal and hyperresponsive airways are unclear, it has been suggested that the phenomenon is related to changes in ASM adaptability to mechanical oscillation. Healthy immature airways of both human and animal exhibit hyperresponsiveness, but whether the adaptative properties of hyperresponsive airway differ from normal is still unknown. In this article, we review the phenomenon of ASM adaptation to mechanical oscillation and its relevance and implication to airway hyperresponsiveness. We demonstrate that the age-specific expression of ASM adaptation is prominent using an established maturational animal model developed in our laboratory. Our data on immature ASM showed potentiated contractile force shortly after a length oscillation compared with the maximum force generated before oscillation. Several potential mechanisms such as myogenic response, changes in actin polymerization, or changes in the quantity of the cytoskeletal regulatory proteins plectin and vimentin, which may underlie this age-specific force potentiation, are discussed. We suggest a working model of the structure of smooth muscle associated with force transmission, which may help to elucidate the mechanisms responsible for the age-specific expression of smooth muscle adaptation. It is important to study the maturational profile of ASM adaptation as it could contribute to juvenile hyperresponsiveness.Key words:ASM adaptation, maturation, bronchoprotection, airway hyperresponsiveness.