Resolution of human mercapt- and nonmercaptalbumin by high-performance liquid chromatography.
Resolution of human mercapt- and nonmercaptalbumin by high-performance liquid chromatography.
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通过高效液相色谱法分离人硫醇和非硫醇白蛋白。
DOI：
10.1111/j.1399-3011.1984.tb00933.x
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发表时间：
2009
期刊：
影响因子：
--
通讯作者：
K. Noguchi
中科院分区：
文献类型：
--
作者：
M. Sogami;S. Nagoka;S. Era;M. Honda;K. Noguchi
在PGP 2000色谱柱（0.10 M磷酸钠缓冲液，0.30 M NaCl, pH 6.86）上对人血清白蛋白（HSA）进行凝胶排除高效液相色谱（HPLC）分析，发现至少有两个峰，主成分对应人巯基白蛋白（HMA），第二峰对应人非巯基白蛋白（HNA）。HMA与HNA分离的机制可能是由于树脂- hsa的弱相互作用。HPLC分析牛血浆白蛋白（BPA）在PGP 2000柱上呈单峰。HSA的洗脱体积大于BPA的洗脱体积，使得HSA和BPA的分离更加清晰。
Gel-exclusion high-performance liquid chromatographic (HPLC) analysis of human serum albumin (HSA) on PGP 2000 column (0.10 M sodium phosphate buffer, 0.30 M NaCl, pH 6.86) showed at least two peaks, the principal component corresponding to human mercaptalbumin (HMA) and the second one to human nonmercaptalbumin (HNA). Mechanism for the separation of HMA and HNA might be due to weak resin-HSA interaction. HPLC analysis of bovine plasma albumin (BPA) showed a single peak on PGP 2000 column. The elution volume of HSA was larger than that of BPA, resulting in a clear resolution of HSA and BPA.