Prf, a novel Ets family protein that binds to the PU.1 binding motif, is specifically expressed in restricted stages of B cell development
Prf, a novel Ets family protein that binds to the PU.1 binding motif, is specifically expressed in restricted stages of B cell development
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DOI：
10.1093/intimm/11.9.1423
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发表时间：
1999-09-01
影响因子：
4.4
通讯作者：
Sakano, H
中科院分区：
文献类型：
--
作者：
Hashimoto, S;Nishizumi, H;Sakano, H
在淋巴细胞发育过程中，IG基因的表达以组织特异性方式和时间顺序方式受到严格调控。我们先前已经表明，在IG kappa 3'增强子（kappa E3'）中的PU. 1结合基序和除PU. 1之外的新Ets家族蛋白可能参与控制V-κ-J（κ）连接。为了分离新的Ets家族蛋白，我们用酵母单杂交方法筛选cDNA文库，并鉴定了一个新的PU.1相关因子Prf。该新的Ets家族蛋白被证明与包括kappa E3 '在内的各种启动子和增强子中的PU.1结合序列相互作用。发现prf基因在B系细胞中的表达占优势，除了未成熟的A细胞。由于Prf不具有转录活性，因此该蛋白可能是Ets家族的其他蛋白如PU.1和Spi-B的拮抗剂。本文还讨论了Prf在B细胞分化中的可能作用。
During the development of lymphocytes, expression of the Ig genes is strictly regulated in a tissue-specific manner and in a time-ordered fashion. We have previously shown that the PU.1 binding motif in the Ig kappa 3' enhancer (kappa E3') and a novel Ets family protein other than PU.1 may be possibly involved in the control of V-kappa-J(kappa) joining. In the attempt to isolate the novel Ets family protein, we have screened cDNA libraries with the yeast one-hybrid method and identified a new PU.1-related factor, Prf, This novel Ets family protein is shown to interact with the PU.1 binding sequences in various promoters and enhancers, including kappa E3', It was found that expression of the prf gene is predominant in the B-lineage cells, with the exception of immature a cells. Since Prf does not exhibit functions of transcriptional activity, this novel protein may act as an antagonist against other Ets family proteins, e.g. PU.1 and Spi-B, Possible roles of Prf with respect to the B cell differentiation are discussed.