Neutral ceramidase-enriched exosomes prevent palmitic acid-induced insulin resistance in H4IIEC3 hepatocytes.
Neutral ceramidase-enriched exosomes prevent palmitic acid-induced insulin resistance in H4IIEC3 hepatocytes.
复制标题
富含中性神经酰胺酶的外泌体可预防 H4IIEC3 肝细胞中棕榈酸诱导的胰岛素抵抗
DOI：
10.1002/2211-5463.12125
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发表时间：
2016-11
期刊：
影响因子：
2.6
通讯作者：
Jin, Junfei
中科院分区：
文献类型：
--
作者：
Zhu, Qun;Zhu, Rongping;Jin, Junfei
游离脂肪酸如棕榈酸（PA）从门静脉供过于求可引起肝脏胰岛素抵抗。活性氧的产生在PA诱导的H4IIEC3肝细胞胰岛素抵抗中起关键作用。最近，我们发现从转染中性神经酰胺酶（NCDase）质粒的INS - 1细胞分泌的外泌体提高了NCDase的活性；这些富集ncase的外泌体可以抑制PA诱导的INS - 1细胞凋亡。在这里，我们发现PA减少了胰岛素刺激的胰岛素受体底物2的酪氨酸磷酸化，并减少了胰岛素刺激的荧光葡萄糖类似物2 - NBDG的摄取，证实了PA处理的H4IIEC3细胞发生了胰岛素抵抗。此外，来自INS - 1细胞的富含ncase的外泌体挽救了PA诱导的H4IIEC3胰岛素抵抗，并阻断了PA诱导的与神经酰胺有关的活性氧的产生。
Oversupply of free fatty acids such as palmitic acid (PA) from the portal vein may cause liver insulin resistance. Production of reactive oxygen species plays a pivotal role in PA‐induced insulin resistance in H4IIEC3 hepatocytes. Recently, we found that exosomes secreted from INS‐1 cells that were transfected with neutral ceramidase (NCDase) plasmids had raised NCDase activity; these NCDase‐enriched exosomes could inhibit PA‐induced INS‐1 cell apoptosis. Here, we showed that PA reduced insulin‐stimulated tyrosine phosphorylation of insulin receptor substrate 2 and decreased insulin‐stimulated uptake of the fluorescent glucose analog 2‐NBDG, confirming that insulin resistance occurred in PA‐treated H4IIEC3 cells. Moreover, NCDase‐enriched exosomes from INS‐1 cells rescued PA‐induced H4IIEC3 insulin resistance and blocked PA‐induced reactive oxygen species production in which ceramide was involved.
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DOI：
10.1152/ajpendo.00251.2013
发表时间：
2013-10-01
影响因子：
5.1
作者：
Jin, Junfei;Zhang, Xiaoming;Huang, Yan
通讯作者：
Huang, Yan
DOI：
10.1590/1414-431x20144238
发表时间：
2015-05
期刊：
Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologicas
影响因子：
--
作者：
Li HB;Yang YR;Mo ZJ;Ding Y;Jiang WJ
通讯作者：
Jiang WJ
DOI：
10.1165/ajrcmb.24.6.4228
发表时间：
2001-06-01
影响因子：
6.4
作者：
Corda, S;Laplace, C;Duranteau, J
通讯作者：
Duranteau, J
影响因子：
8.2
作者：
Zhu, Qun;Jin, Jun-fei;Liu, Chao
通讯作者：
Liu, Chao
神经酰胺在肝胰岛素抵抗发展中作用的新证据。
影响因子：
3.7
作者：
Konstantynowicz-Nowicka K;Harasim E;Baranowski M;Chabowski A
通讯作者：
Chabowski A