Preliminary crystallographic study of two cuticle-degrading proteases from the nematophagous fungi Lecanicillium psalliotae and Paecilomyces lilacinus.
Preliminary crystallographic study of two cuticle-degrading proteases from the nematophagous fungi Lecanicillium psalliotae and Paecilomyces lilacinus.
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来自食线虫真菌 Lecanicillium psalliotae 和 Paecilomyces lilacinus 的两种角质层降解蛋白酶的初步晶体学研究。
DOI：
10.1107/s1744309109003595
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发表时间：
2009-03
期刊：
影响因子：
--
通讯作者：
孟照辉
中科院分区：
文献类型：
--
作者：
孟照辉
角质层降解蛋白酶是细胞外枯草杆菌蛋白酶样丝氨酸蛋白酶，由昆虫病原真菌和食线虫真菌分泌。这些蛋白酶可以在昆虫或线虫入侵过程中消化宿主角质层，并在食线虫真菌感染线虫过程中充当一组重要的毒力因子。为了阐明蛋白酶和线虫角质层之间的相互作用机制，研究了两种角质层降解蛋白酶：来自 Lecanicillium psalliotae（同名 Verticillium psalliotae）的 Ver112 和来自淡紫拟青霉（Paecilomyces lilacinus）的 PL646。使用悬滴蒸气扩散法在 289 K 下使 Ver112 蛋白和 PL646 与底物样四肽抑制剂甲氧基琥珀酰-Ala-Ala-Pro-Val-氯甲基酮 (MSU-AAPV) 之间的复合物结晶。通过 X 射线衍射分析晶体，分辨率分别为 1.65 和 2.2 A。这些分析表明，Ver112 晶体属于空间群 P2(1)2(1)2(1)，晶胞参数 a = 43.7，b = 67.8，c = 76.3 A，α = beta = gamma = 90 度。相比之下，PL646-MSU-AAPV复合物的晶体属于P2(1)空间群，晶胞参数a = 65.1，b = 62.5，c = 67.6 A，β = 92.8度。
Cuticle-degrading proteases are extracellular subtilisin-like serine proteases that are secreted by entomopathogenic and nematophagous fungi. These proteases can digest the host cuticle during invasion of an insect or nematode and serve as a group of important virulence factors during the infection of nematodes by nematophagous fungi. To elucidate the mechanism of interaction between the proteases and the nematode cuticle, two cuticle-degrading proteases, Ver112 from Lecanicillium psalliotae (syn. Verticillium psalliotae) and PL646 from Paecilomyces lilacinus, were studied. The Ver112 protein and the complex between PL646 and the substrate-like tetrapeptide inhibitor methoxysuccinyl-Ala-Ala-Pro-Val-chloromethyl ketone (MSU-AAPV) were crystallized using the hanging-drop vapour-diffusion method at 289 K. The crystals were analyzed by X-ray diffraction to resolutions of 1.65 and 2.2 A, respectively. These analyses identified that crystals of Ver112 belonged to space group P2(1)2(1)2(1), with unit-cell parameters a = 43.7, b = 67.8, c = 76.3 A, alpha = beta = gamma = 90 degrees . In contrast, crystals of the PL646-MSU-AAPV complex belonged to space group P2(1), with unit-cell parameters a = 65.1, b = 62.5, c = 67.6 A, beta = 92.8 degrees .
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影响因子：
2.7
作者：
Jinkui Yang;Xiaowei Huang;B. Tian;Miao Wang;Qiuhong Niu;Keqin Zhang
通讯作者：
Jinkui Yang;Xiaowei Huang;B. Tian;Miao Wang;Qiuhong Niu;Keqin Zhang
影响因子：
2.8
作者：
L. V. Lopez-Llorca
通讯作者：
L. V. Lopez-Llorca
影响因子：
2.8
作者：
SEGERS, R;BUTT, TM;PEBERDY, JF
通讯作者：
PEBERDY, JF
影响因子：
2.7
作者：
Jinkui Yang;Xiaowei Huang;B. Tian;Hui Sun;J. Duan;Wenping Wu;Keqin Zhang
通讯作者：
Jinkui Yang;Xiaowei Huang;B. Tian;Hui Sun;J. Duan;Wenping Wu;Keqin Zhang
DOI：
10.1016/0147-5975(92)90002-9
发表时间：
1992-12
期刊：
Experimental Mycology
影响因子：
--
作者：
L. V. Lopez-Llorca;W. M. Robertson
通讯作者：
L. V. Lopez-Llorca;W. M. Robertson