Design, Recombinant Expression, and Antibacterial Activity of the Cecropins-Melittin Hybrid Antimicrobial Peptides
Design, Recombinant Expression, and Antibacterial Activity of the Cecropins-Melittin Hybrid Antimicrobial Peptides
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天蚕素-蜂毒肽杂合抗菌肽的设计、重组表达和抗菌活性
DOI：
10.1007/s00284-010-9592-7
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发表时间：
2010-09-01
影响因子：
2.6
通讯作者：
Qiao, Dai Rong
中科院分区：
文献类型：
--
作者：
Cao, Yu;Yu, Rong Qing;Qiao, Dai Rong
为了评价其抗菌活性和毒性，利用APD2数据库设计了天蚕素-蜂毒肽混合抗菌肽CA(1-7)-M(4-11) (CAM)和CB(1-7)-M(4-11) (CBM)。重组杂合抗菌肽在毕赤酵母中成功表达和纯化。抗菌活性测定表明，两种混合抗菌肽对大肠杆菌、金黄色葡萄球菌、铜绿假单胞菌、肺炎克雷伯菌、枯草芽孢杆菌、苏云金芽孢杆菌和德比沙门氏菌均具有较强的抗菌能力。 CAM和CBM对大肠杆菌25922的效价分别为0.862和0.849，略低于Amp的0.957。溶血实验表明CAM和CBM在体内外均无溶血现象，具有良好的应用前景。
In order to evaluate their antibacterial activities and toxicities, the cecropins-melittin hybrid antimicrobial peptide, CA(1-7)-M(4-11) (CAM) and CB(1-7)-M(4-11) (CBM), were designed by APD2 database. The recombinant hybrid antimicrobial peptides were successfully expressed and purified in Pichia pastoris. Antimicrobial activity assay showed that both of the two hybrid antimicrobial peptides had strong antibacterial abilities against Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa, Klebsiella pneumoniae, Bacillus subtilis, Bacillus thuringiensis, and Salmonella derby. The potency of CAM and CBM to E. coli 25922 were 0.862 and 0.849, respectively, slightly lower than Amp's 0.957. The hemolytic assays indicated CAM and CBM had no hemolytic in vivo and in vitro, and so they had a good application prospect.