Three-dimensional interferometric scattering microscopy via remote focusing technique
Three-dimensional interferometric scattering microscopy via remote focusing technique
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DOI：
10.1364/ol.386172
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发表时间：
2020-05-01
期刊：
影响因子：
3.6
通讯作者：
Cho, Minhaeng
中科院分区：
文献类型：
--
作者：
Lee, Il-buem;Moon, Hyeon-Min;Cho, Minhaeng
干涉散射（iSCAT）显微镜使我们能够以高的空间和时间分辨率跟踪nm大小的物体，并允许生物分子的无标记成像。然而，它卓越的灵敏度是以几个缺点为代价的，比如三维成像速度慢和垂直跟踪有限。在这里，我们提出了一种新的方法，远程聚焦iSCAT（RF-iSCAT）显微镜，可视化的体积标本成像部分在不同的深度，没有翻译的目标透镜或样品阶段。我们通过平移参考镜来确定亚微米微珠的z位置，从而演示了RF-iSCAT的原理。RF-iSCAT具有前所未有的长垂直跟踪范围，允许快速但无振动的垂直扫描。我们预计，RF-iSCAT将提高iSCAT的动力学研究的效用。(C)2020美国光学学会
Interferometric scattering (iSCAT) microscopy enables us to track nm-sized objects with high spatial and temporal resolutions and permits label-free imaging of biomolecules. Its superb sensitivity, however, comes at a cost by several downsides, such as slow three-dimensional imaging and limited vertical tracking. Here, we propose a new method, Remote Focusing-iSCAT (RF-iSCAT) microscopy, to visualize a volume specimen by imaging sections at different depths without translation of either the objective lens or sample stage. We demonstrate the principle of RF-iSCAT by determining the z-position of submicrometer beads by translating the reference mirror instead. RF-iSCAT features an unprecedentedly long range of vertical tracking and permits fast but vibration-free vertical scanning. We anticipate that RF-iSCAT would enhance the utility of iSCAT for dynamics study. (C) 2020 Optical Society of America