Histone variants expression in peripheral blood mononuclear cells of patients with rheumatoid arthritis
Histone variants expression in peripheral blood mononuclear cells of patients with rheumatoid arthritis
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DOI：
10.1111/1756-185x.13126
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发表时间：
2018-10-01
影响因子：
2.5
通讯作者：
Mahmoudi, Mahdi
中科院分区：
文献类型：
--
作者：
Asadipour, Maryam;Hassan-Zadeh, Vahideh;Mahmoudi, Mahdi
目的：本研究的目的是分析类风湿关节炎患者和健康对照者外周血单核细胞（PBMC）样本中涉及基因表达调控的四种组蛋白变异的表达。方法：分析组蛋白变异H3.3、H2A三个基因的表达。采用SYBR绿色实时聚合酶链反应检测50例根据美国风湿病学会（American College of Rheumatology， ACR）标准诊断的RA患者PBMC样本中的Z、macroH2A1.1和macroH2A1.2，以及51例匹配的健康对照。采用Mann-Whitney u检验和Spearman相关进行数据分析。结果：H2A的表达；与健康对照相比，RA患者PBMCs中Z表达增加1.51倍（P < 0.001）， H3.3表达增加1.13倍（P = 0.048）。此外，我们发现基于红细胞沉降率的疾病活动评分（DAS-28）与H2A.Z的表达呈正相关。结论：考虑到H3.3和H2A的作用。在核小体定位、染色质结构和转录调控方面，我们认为淋巴细胞和单核细胞是RA患者pbmc的主要细胞亚型，它们拥有更容易接近的染色质。
Aim: The purpose of the present study was to analyze the expression of four histone variants, implicated in the regulation of gene expression, in peripheral blood mononuclear cell (PBMC) samples of patients with rheumatoid arthritis and healthy controls.Method: We analyzed the expression of three genes encoding histone variants H3.3, H2A.Z, macroH2A1.1 and macroH2A1.2 in PBMC samples of 50 patients with RA, diagnosed according to American College of Rheumatology (ACR) criteria, and 51 matched healthy controls using SYBR green real-time polymerase chain reaction. Mann-Whitney U-test and Spearman correlation were used for data analysis.Results: The expression of H2A.Z was increased by 1.51-fold (P < 0.001) and the expression of H3.3 was increased by 1.13-fold (P = 0.048) in PBMCs of patients with RA compared to healthy controls. Furthermore, we found a positive correlation between Disease Activity Score (DAS-28) based on erythrocyte sedimentation rate and the expression of H2A.Z.Conclusions: Given the role of H3.3 and H2A.Z in nucleosome positioning, chromatin structure and transcription regulation, we suggest that lymphocytes and monocytes, the main cell subtypes in PBMCs of RA patients, possess a more accessible chromatin.