Expression of Aspergillus fumigatus virulence-related genes detected in vitro and in vivo with competitive RT-PCR.
Expression of Aspergillus fumigatus virulence-related genes detected in vitro and in vivo with competitive RT-PCR.
复制标题
通过竞争性 RT-PCR 检测烟曲霉毒力相关基因的体外和体内表达。
DOI：
10.1007/s11046-005-0141-z
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发表时间：
2005
期刊：
影响因子：
5.5
通讯作者：
Calderone,Richard
中科院分区：
文献类型：
--
作者：
Zhang,Lijuan;Wang,Mingyue;Li,Ruoyu;Calderone,Richard
我们已经测量了几个基因的转录，这些基因被认为是烟曲霉中的毒力因子，包括fos-1，一种组氨酸激酶，双组分信号蛋白，rhbA，一种信号传导所需的ras相关蛋白，pksP，一种参与黑色素生物合成的聚酮合酶，pabA合成酶，一种催化叶酸生物合成后期步骤的酶，lysF，一种与赖氨酸生物合成相关的高壳聚糖酶，以及cpcA，氨基酸生物合成交叉途径控制系统的转录激活因子。从体外培养的生物体以及从感染A.烟熏。我们的数据表明，在小鼠感染过程中，fos-1和rhbA的转录显著增加，与其他转录保持不变（pksP，cpcA，pabA）或略有下降（lysF）的基因相比，在感染的肺组织中，体外转录的测量显示，与转录相比，fos-1和rhbA的水平较低，增加20-40倍（cpcA，lysF，pabA），而在培养物中未检测到pksP。我们的数据表明，这些基因的转录在体外与疾病期间不同。
We have measured the transcription of several genes that have been implicated as virulence factors inAspergillus fumigatus, includingfos-1, a histidine kinase, two-component signal protein,rhbA, a ras-related protein required for signaling,pksP, a polyketide synthase involved in biosynthesis of melanin,pabAsynthetase, an enzyme catalyzing a late step in the biosynthesis of folate,lysF, a homoconitase related to lysine biosynthesis, andcpcA, the transcriptional activator of the cross-pathway control system of amino acid biosynthesis. Transcription levels were determined fromin vitrogrown organism as well as from lung tissue from mice infected withA. fumigatus. Our data indicate thatfos-1andrhbAtranscription increased significantly during the infection in mice, compared to the other genes whose transcription remained the same (pksP, cpcA, pabA) or decreased slightly (lysF).In vitromeasurements of transcription compared to transcription in infected lung tissue demonstrated low levels offos-1andrhbA, 20–40-fold increases (cpcA, lysF, pabA), whilepksPwas not detected from cultures. Our data demonstrate that transcription of these genes differsin vitroversus during disease.