Dense connectomic reconstruction in layer 4 of the somatosensory cortex
Dense connectomic reconstruction in layer 4 of the somatosensory cortex
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DOI：
10.1126/science.aay3134
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发表时间：
2019-11-29
期刊：
影响因子：
56.9
通讯作者：
Helmstaedter, Moritz
中科院分区：
文献类型：
--
作者：
Motta, Alessandro;Berning, Manuel;Helmstaedter, Moritz
哺乳动物大脑皮层的致密回路结构仍然未知。随着三维电子显微镜的发展，对相当大体积的神经纤维进行成像已经成为可能，但连接组的密集重建是限制步骤。我们从小鼠桶状皮层第 4 层重建了大约 500,000 立方微米的体积，比之前从哺乳动物大脑皮层进行的密集重建大了 300 倍。连接组数据允许提取无法从几何信息预测的抑制性和兴奋性神经元亚型。我们量化了与赫布突触权重适应一致的连接组印记，这产生了与饱和长期增强一致的电路部分的上限。这些数据建立了一种对哺乳动物皮层神经元回路进行局部致密连接体表型分析的方法。
The dense circuit structure of mammalian cerebral cortex is still unknown. With developments in three-dimensional electron microscopy, the imaging of sizable volumes of neuropil has become possible, but dense reconstruction of connectomes is the limiting step. We reconstructed a volume of similar to 500,000 cubic micrometers from layer 4 of mouse barrel cortex, similar to 300 times larger than previous dense reconstructions from the mammalian cerebral cortex. The connectomic data allowed the extraction of inhibitory and excitatory neuron subtypes that were not predictable from geometric information. We quantified connectomic imprints consistent with Hebbian synaptic weight adaptation, which yielded upper bounds for the fraction of the circuit consistent with saturated long-term potentiation. These data establish an approach for the locally dense connectomic phenotyping of neuronal circuitry in the mammalian cortex.