NG2-positive cells generate A2B5-positive oligodendrocyte precursor cells
NG2-positive cells generate A2B5-positive oligodendrocyte precursor cells
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DOI：
10.1002/glia.20519
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发表时间：
2007-08-01
期刊：
影响因子：
6.2
通讯作者：
Trapp, Bruce D.
中科院分区：
文献类型：
--
作者：
Baracskay, Karen L.;Kidd, Grahame J.;Trapp, Bruce D.
少突胶质细胞谱系的细胞特化经历了一系列通过不同生化特征的表达来识别的阶段。体外特征最好的少突胶质细胞祖细胞 (OPC) 是双能 O2-A 祖细胞，通过单克隆抗体 A2B5 标记来鉴定，其增殖主要响应血小板衍生生长因子 (PDGF)。 O2-A 祖细胞的细胞祖先目前尚不清楚。体内 OPC 可以通过细胞表面标志物 NG2（一种硫酸化蛋白聚糖）和血小板衍生生长因子受体 α (PDGF α R) 的表达进行鉴定。大量证据支持在体内和体外从 NG2(+)、PDGF α R+ 细胞产生少突胶质细胞。 NG2(+)细胞和A2B5(-)阳性细胞之间的发育关系尚不清楚，也不清楚它们是否代表相同、部分重叠或不重叠的细胞群。在这里，我们表明，在发育中的大脑培养物中，NG2（+）和A2B5（+）细胞来自重叠的细胞群。 NG2(+) 细胞先于 A2B5(+) 细胞表达并生成 A2B5(-) 细胞（前 OPC），代表 A2B5(+) O2A 祖细胞 (OPC) 的直接祖先。 (c) 2007 年 Wiley-Liss, Inc.
Cellular specification of the oligodendrocyte lineage occurs through a series of stages identified by expression of distinct biochemical characteristics. The best characterized oligodendrocyte progenitor cell (OPC) in vitro is the bipotential O2-A progenitor, identified by labeling with monoclonal antibody A2B5, which proliferates predominantly in response to platelet derived growth factor (PDGF). The cellular ancestors of O2-A progenitor cells are currently unclear. In vivo OPCs can be identified by expression of the cell surface markers NG2 (a sulfated proteoglycan) and platelet derived growth factor receptor alpha (PDGF alpha R). Substantial evidence supports the generation of oligodendrocytes from NG2(+), PDGF alpha R+ cells both in vivo and in vitro. The developmental relationship between NG2(+) cells and A2B5(-) positive cells is unknown and it is unclear whether they represent identical, partially overlapping or nonoverlapping populations of cells. Here we show that in cultures of developing brain NG2(+) and A2B5(+) cells arise from overlapping cell populations. NG2(+) cells appear prior to the expression of A2B5(+) cells and generate A2B5(-) cells (pre-OPCs) represent the direct ancestor to A2B5(+) O2A progenitor cells (OPCs). (c) 2007 Wiley-Liss, Inc.