Protein adsorption and cellular uptake of cerium oxide nanoparticles as a function of zeta potential
Protein adsorption and cellular uptake of cerium oxide nanoparticles as a function of zeta potential
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DOI：
10.1016/j.biomaterials.2007.07.029
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发表时间：
2007-11-01
期刊：
影响因子：
14
通讯作者：
Seal, Sudipta
中科院分区：
文献类型：
--
作者：
Patil, Swanand;Sandberg, Amanda;Seal, Sudipta
生物材料的表面化学对其在生物应用中的性能具有显著影响。我们最近的工作表明，氧化铈纳米颗粒是细胞培养模型中有效的抗氧化剂，我们已经评估了这些纳米颗粒在不同生物系统中的几种治疗应用。蛋白质吸附和细胞摄取的知识将是非常有用的，在提高氧化铈纳米粒子在生物学中的有益效果。在本研究中，我们确定了氧化铈纳米粒子的zeta电位对牛血清白蛋白（BSA）的吸附和肺腺癌细胞（A549）的细胞摄取的影响。通过将纳米颗粒分散在各种酸性和碱性pH溶液中来改变纳米颗粒的zeta电位。紫外可见光谱法和电感耦合等离子体质谱法（ICP-MS）分别用于蛋白质吸附和细胞摄取研究。发现具有正zeta电位的纳米氧化铈样品吸附更多的BSA，而具有负zeta电位的样品显示很少或没有蛋白质吸附。细胞吸收研究表明，带负电荷的纳米颗粒优先吸收。这些结果表明，静电相互作用可以在纳米颗粒的蛋白质吸附和细胞摄取中发挥重要作用。爱思唯尔有限公司出版
The surface chemistry of biomaterials can have a significant impact on their performance in biological applications. Our recent work suggests that cerium oxide nanoparticles are potent antioxidants in cell culture models and we have evaluated several therapeutic applications of these nanoparticles in different biological systems. Knowledge of protein adsorption and cellular uptake will be very useful in improving the beneficial effects of cerium oxide nanoparticles in biology. In the present study, we determined the effect of zeta potential of cerium oxide nanoparticles on adsorption of bovine serum albumin (BSA) and cellular uptake in adenocarcinoma lung cells (A549). The zeta potential of the nanoparticles was varied by dispersing them in various acidic and basic pH solutions. UV-visible spectroscopy and inductively coupled plasma mass spectrometry (ICP-MS) were used for the protein adsorption and cellular uptake studies, respectively. Nanoceria samples having positive zeta potential were found to adsorb more BSA while the samples with negative zeta potential showed little or no protein adsorption. The cellular uptake studies showed preferential uptake for the negatively charged nanoparticles. These results demonstrate that electrostatic interactions can play an important factor in protein adsorption and cellular uptake of nanoparticles. Published by Elsevier Ltd.