Profile of acute immune response in Chinese amphioxus upon Staphylococcus aureus and Vibrio parahaemolyticus infection
Profile of acute immune response in Chinese amphioxus upon Staphylococcus aureus and Vibrio parahaemolyticus infection
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DOI：
10.1016/j.dci.2007.01.003
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发表时间：
2007-01-01
影响因子：
2.9
通讯作者：
Xu, Anlong
中科院分区：
文献类型：
--
作者：
Huang, Gonghua;Liu, Hui;Xu, Anlong
文昌鱼传统上被认为是现存的无脊椎动物，与脊椎动物关系最密切。然而，关于文昌鱼如何防御微生物入侵的系统研究还没有进行。本文报道了金黄色葡萄球菌(S.c)和副溶血性弧菌(V.P)感染后，抑制消减杂交(SSH)后的基因转录图谱。与哺乳动物相比，文昌鱼具有相同的急性免疫防御基因(凝集素、金属蛋白酶、溶菌酶和抗菌肽等)。以及相似的时间基因表达模式和水平。相反，文昌鱼还发现了一些响应微生物攻击的新的急性免疫反应基因，如apexpin和dermatopontin，它们在弧菌感染后分别有3500倍和900倍的诱导，这表明这两个基因在早期免疫系统中具有新的功能。我们的结果首次报道了原索动物原始免疫系统对感染的防御情况。(C)2007年由爱思唯尔有限公司出版。
Amphioxus is traditionally considered as the living invertebrate most closely related to vertebrate. However, no systematic study was performed about how the amphioxus defends against the microbial invasion. Here we reported a profile of gene transcription after Staphylococcus aureus (S. c) and Vibrio parahaemolyticus (V.p) challenged by suppression subtractive hybridization (SSH). When compared with mammals, amphioxus has the same acute immune defense genes (lectins, metalloproteinase, lysozymes and antimicrobial peptide, etc.) as well as a similar pattern and level of temporal gene expression. In contrast, amphioxus was demonstrated to have some novel acute immune response genes in response to the microbial challenge, such as apextrin and dermatopontin, which have a 3500-fold and 900-fold induction after the V.p infection, respectively, suggesting new functions in early immune system for these two genes. Our results reported for the first time a profile of primitive immune system defense against infection in protochordate. (C) 2007 Published by Elsevier Ltd.