Genome sequence of Yersinia pestis, the causative agent of plague
Genome sequence of Yersinia pestis, the causative agent of plague
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DOI：
10.1038/35097083
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发表时间：
2001-10-04
期刊：
影响因子：
64.8
通讯作者：
Barrell, BG
中科院分区：
文献类型：
--
作者：
Parkhill, J;Wren, BW;Barrell, BG
革兰氏阴性菌鼠疫耶尔森氏菌是全身性侵入性传染病的病原体，经典上称为鼠疫（1），并导致了三次人类大流行病：查士丁尼鼠疫（6至8世纪），黑死病（14至19世纪）和现代鼠疫（世纪至今）。本文综述了近年来耐多药菌株的鉴定及应用前景。鼠疫作为一种生物战剂，意味着鼠疫仍然对人类健康构成威胁。在这里，我们报告的完整基因组序列的Y。鼠疫菌CO92株由4.65Mb染色体和96.2kb、70.3kb和9.6kb质粒组成。基因组中异常丰富的插入序列，并显示异常的GC碱基组成的偏见，表明频繁的基因组内重组。许多基因似乎是从其他细菌和病毒获得的（包括粘附素、分泌系统和杀虫毒素）。基因组包含大约150个假基因，其中许多是多余的肠病生活方式的残余。基因组的流动性、扩张和衰退的证据表明Y。鼠疫是一种经历了大规模基因流动的病原体，它为了解新的和高毒性病原体的演变方式提供了独特的见解。
The Gram-negative bacterium Yersinia pestis is the causative agent of the systemic invasive infectious disease classically referred to as plague(1), and has been responsible for three human pandemics: the Justinian plague (sixth to eighth centuries), the Black Death (fourteenth to nineteenth centuries) and modern plague (nineteenth century to the present day). The recent identification of strains resistant to multiple drugs(2) and the potential use of Y. pestis as an agent of biological warfare mean that plague still poses a threat to human health. Here we report the complete genome sequence of Y. pestis strain CO92, consisting of a 4.65-megabase (Mb) chromosome and three plasmids of 96.2 kilobases (kb), 70.3 kb and 9.6 kb. The genome is unusually rich in insertion sequences and displays anomalies in GC base-composition bias, indicating frequent intragenomic recombination. Many genes seem to have been acquired from other bacteria and viruses (including adhesins, secretion systems and insecticidal toxins). The genome contains around 150 pseudogenes, many of which are remnants of a redundant enteropathogenic lifestyle. The evidence of ongoing genome fluidity, expansion and decay suggests Y. pestis is a pathogen that has undergone large-scale genetic flux and provides a unique insight into the ways in which new and highly virulent pathogens evolve.