EVOLUTION OF HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1 NUCLEOTIDE-SEQUENCE DIVERSITY AMONG CLOSE CONTACTS
EVOLUTION OF HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1 NUCLEOTIDE-SEQUENCE DIVERSITY AMONG CLOSE CONTACTS
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DOI：
10.1073/pnas.88.24.11236
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发表时间：
1991-12-01
影响因子：
11.1
通讯作者：
GIBBS, RA
中科院分区：
文献类型：
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作者：
BURGER, H;WEISER, B;GIBBS, RA
人类免疫缺陷病毒1型（HIV-1）通过性行为从一个个体传播给另一个个体或从母亲垂直传播给孩子时，其核苷酸序列的变化程度尚不清楚。先前的研究表明，来自同一个体的大多数培养的HIV-1分离株在整个包膜基因核苷酸序列上存在高达2%的差异，尽管来自无关个体的大多数分离株存在6- 22%的差异。为了检验来自密切接触者的HIV-1分离株的多样性，我们确定了来自一个已知流行病学概况的家庭的病毒的核苷酸序列，其中一名妇女通过异性恋将HIV-1传播给她的伴侣，并垂直传播给她的女儿。对PCR扩增的HIV-1原代分离株进行直接DNA序列分析，区分主、次病毒序列（称为准种），快速确定优势序列及其系统发育关系。HIV-1包膜基因主要部分的核苷酸序列多样性在该妇女及其异性伴侣的分离株中为3.7%，在该妇女及其比其伴侣感染时间更长的女儿的分离株中为8.5%。系统发育树的结构表明，女儿的主要分离物是从她母亲当前菌株的一个祖先进化而来的。这项研究提供了证据，证明任何两个分离株之间的序列多样性都是连续的，从来自同一个人的分离株到来自密切接触者的分离株，最后是来自无关个体的分离株。这些数据和方法可用于卫生保健工作者与其患者之间可能的HIV-1传播的流行病学调查。
The degree of change in the nucleotide sequence of human immunodeficiency virus type 1 (HIV-1) that occurs when it is transmitted sexually from one individual to another or vertically from mother to child is unknown. Previous studies have shown that most cultured HIV-1 isolates from the same individuals differed in the entire envelope gene nucleotide sequence by up to 2%, although most isolates from unrelated individuals differed by 6-22 %. To examine diversity among HIV-1 isolates from close contacts, we determined the nucleotide sequences of viruses from a family with a known epidemiologic profile, in which a woman transmitted HIV-1 heterosexually to her partner and vertically to her daughter. Direct DNA sequence analysis of primary HIV-1 isolates amplified by PCR was used to distinguish the major and minor viral sequences, termed quasispecies, to rapidly determine the predominant sequences and their phylogenetic relationships. The nucleotide sequence diversity of a major portion of the HIV-1 envelope gene was 3.7% between isolates from the woman and her heterosexual partner and 8.5% between isolates from this woman and her daughter, who had been infected for a longer period than the partner. The configuration of the phylogenetic tree demonstrated that the daughter's predominant isolate evolved from a progenitor of her mother's current strain. This study provides evidence of a continuous spectrum of sequence diversity between any two isolates ranging from those derived from the same person to those from close contacts and, ultimately, those from unrelated individuals. These data and methods can be applied to epidemiologic investigations of possible HIV-1 transmission between health care workers and their patients.