Effect of Jieduhuayu Granule on the Expression of Caspase-3mRNA in Liver Cells of Acute Liver Failure Mice
Effect of Jieduhuayu Granule on the Expression of Caspase-3mRNA in Liver Cells of Acute Liver Failure Mice
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发表时间：
2009
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通讯作者：
Long Fu-li
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作者：
Long Fu-li
目的探讨解毒化瘀颗粒对急性肝衰竭小鼠肝细胞凋亡的影响及其可能机制。采用D-GalN+LPS腹腔注射建立小鼠急性肝衰竭模型，观察小鼠存活率，SYBR荧光真实的-结果48 h后，解毒化瘀组的存活率高于其他药物组;同时促凋亡因子，Caspase-3在解毒化瘀颗粒组肝组织中的表达较低，而在其他药物治疗组肝组织中的表达较高。结论：Caspase-3表达与急性肝衰竭时肝细胞凋亡有关，解毒化瘀颗粒可通过抑制Caspase-3的表达减轻肝细胞损伤，降低肝衰竭小鼠的死亡率。
Objective To investigate the effect of Jieduhuayu granule on hepatocyte apoptosis in acute liver failure mice and its possible mechanism.MethodsKunming mice were randomly divided into blank control group,model group,and Jieduhuayu treating group.Acute liver failure was built with D-GalN+LPS intraperitoneal injection to the mice.The survival rate was observed and the expression of Caspase-3mRNA was tested with SYBR fluorescence real-time quantitative PCR assay.ResultsAfter 48 h,the survival rate in Jieduhuayu treating group was higher than that of other drug treating group;at the same time pro-apoptotic factor,Caspase-3 expression in liver tissue is low in the Jieduhuayu particle group but high expression in other drug treatment groups.ConclusionThe Caspase-3 expression is related with hepatocyte apoptosis in acute liver failure.Jieduhuayu treatment granule can reduce liver cell injury by through inhibiting the expression of Caspase-3,and reducing mortality rate of liver failure mice.