A novel gene family defined by human dihydropyrimidinase and three related proteins with differential tissue distribution
A novel gene family defined by human dihydropyrimidinase and three related proteins with differential tissue distribution
复制标题
DOI：
10.1016/s0378-1119(96)00445-3
复制
发表时间：
1996-11-21
期刊：
影响因子：
3.5
通讯作者：
Nonaka, M
中科院分区：
文献类型：
--
作者：
Hamajima, N;Matsuda, K;Nonaka, M
我们从人肝脏中分离到了编码二氢嘧啶酶（DHPase）的cDNA克隆，并从人胎儿脑中分离到了其三个同源物。推导出的人DHPase氨基酸序列与大鼠DHPase的同源性为90%，三个同源物与人DHPase的同源性为57 ~ 59%，彼此之间的同源性为74 ~ 77%。我们暂时将这些同源物命名为人类DHPase相关蛋白(DRP)-1、DRP-2和DRP-3。人DRP-2与鸡CRMP-62（相对分子质量为62 kDa的塌陷反应中介蛋白）具有98%的同源性。人DRP-3与大鼠有丝分裂后神经元特异性表达的TOAD-64（分裂后开启，64 kDa）的两个部分肽序列具有94-100%的同源性。人DHPase和DRPs与嗜热脂肪芽孢杆菌hydantoinase（39-42%）和秀丽隐杆线虫unc-33（32-34%）的aa序列一致性较低。因此，我们描述了一个新的基因家族，它显示不同的组织分布：即，人类DHPase，在肝脏和肾脏；人脑DRP-1；人DRP-2，除肝脏外普遍表达；人DRP-3，主要存在于心脏和骨骼肌。
We have isolated cDNA clones encoding dihydropyrimidinase (DHPase) from human liver and its three homologues from human fetal brain. The deduced amino acid (aa) sequence of human DHPase showed 90% identity with that of rat DHPase, and the three homologues showed 57-59% aa identity with human DHPase, and 74-77% aa identity with each other. We tentatively termed these homologues human DHPase related protein (DRP)-1, DRP-2 and DRP-3. Human DRP-2 showed 98% aa identity with chicken CRMP-62 (collapsin response mediator protein of relative molecular mass of 62 kDa) which is involved in neuronal growth cone collapse. Human DRP-3 showed 94-100% aa identity with two partial peptide sequences of rat TOAD-64 (turned on after division, 64 kDa) which is specifically expressed in postmitotic neurons. Human DHPase and DRPs showed a lower degree of aa sequence identity with Bacillus stearothermophilus hydantoinase (39-42%) and Caenorhabditis elegans unc-33 (32-34%). Thus we describe a novel gene family which displays differential tissue distribution: i.e., human DHPase, in liver and kidney; human DRP-1, in brain; human DRP-2, ubiquitously expressed except for liver; human DRP-3, mainly in heart and skeletal muscle.