A Potential Role of Alpha-7 Nicotinic Acetylcholine Receptor in Cardiac Angiogenesis in a Pressure-Overload Rat Model
A Potential Role of Alpha-7 Nicotinic Acetylcholine Receptor in Cardiac Angiogenesis in a Pressure-Overload Rat Model
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Alpha-7 烟碱乙酰胆碱受体在压力超负荷大鼠模型心脏血管生成中的潜在作用
DOI：
10.1254/jphs.09335fp
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发表时间：
2010-11-01
影响因子：
3.5
通讯作者：
Shen, Fu-Ming
中科院分区：
文献类型：
--
作者：
Ni, Min;Yang, Zhong-Wei;Shen, Fu-Ming
本研究通过腹主动脉缩窄引起的压力超负荷大鼠模型，探讨了α 7烟碱乙酰胆碱受体（α 7 nAChR）在左心室的表达及其在心脏血管生成中的作用。在术后4、8、12和16周测量血压和α 7 nAChR蛋白水平。在最后16周测定α 7 nAChR mRNA水平、心脏迷走神经功能、心脏肥大和左心室微血管密度。评价α 7 nAChR在血管生成中的作用。结果发现，缩窄部位以上的收缩压在缩窄后16周时更大，并且与对照组相比，左心室中α 7 nAChR在mRNA和蛋白水平上的表达均显著增加。受体的阳性染色主要集中在血管周围和变性的心肌细胞中。心脏迷走神经功能明显减弱，微血管密度明显增加，并与心脏肥大。激活α 7 nAChR诱导培养的人脐静脉内皮细胞（HUVECs）的管形成。我们的结论是，α 7 nAChR的表达增加缩窄后16周，这可能是一种代偿反应，减少迷走神经功能和心肌肥厚，也可能在心脏血管生成中发挥作用。
This work investigated the expression of alpha-7 nicotinic acetylcholine receptor (alpha 7nAChR) in the left ventricle and its putative role in cardiac angiogenesis in a pressure overload rat model induced by abdominal aorta coarctation. Blood pressure and protein levels of alpha 7nAChR were measured at 4, 8, 12, and 16 weeks after surgery. mRNA levels of alpha 7nAChR, cardiac vagus nerve function, cardiac hypertrophy, and microvessel density of the left ventricle were determined at the final 16-week period. The role of alpha 7nAChR in angiogenesis was evaluated. It was found that systolic blood pressure above the coarctation site was greater at 16 weeks alter coarctation and expression of alpha 7nAChR was significantly increased at both mRNA and protein levels in the left ventricle compared with the control. Positive staining for receptors was mainly focused around vessels and among the degenerated cardiomyocytes. Cardiac vagus nerve function was significantly attenuated; microvessel density was markedly increased and was associated with cardiac hypertrophy. Activation of alpha 7nAChR induced tube formation of cultured human umbilical vein endothelial cells (HUVECs). We conclude that expression of alpha 7nAChR was increased at 16 weeks after coarctation, and this might be a compensatory response to decreased vagus nerve function and cardiac hypertrophy and may also play a role in cardiac angiogenesis.