Proliferating cell nuclear antigen expression in renal cell carcinoma - Prognostic implications
Proliferating cell nuclear antigen expression in renal cell carcinoma - Prognostic implications
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DOI：
10.3109/00365599609182321
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发表时间：
1996-12-01
影响因子：
--
通讯作者：
Ljungberg, B
中科院分区：
文献类型：
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作者：
Larsson, P;Roos, G;Ljungberg, B
增殖细胞核抗原（PCNA）是与活跃细胞周期中的细胞相关的核蛋白。通过免疫组织化学方法评估了 68 例肾细胞癌的 3-6 个样本中 PCNA 的表达。使用单克隆抗体 PC-10 分析了总共 279 个肿瘤样本，并测定了阳性染色细胞核的百分比（PCNA 指数）。肿瘤 PCNA 指数变化范围为 0.9-17.5%，平均值为 4.9%，与正常肾皮质样本显着不同 (p < 0.001)。 PCNA指数在不同肿瘤级别之间存在显着差异，而在临床分期之间没有发现显着差异。关于 DNA 倍性，与二倍体肿瘤相比，非整倍体肿瘤的 PCNA 指数显着较高 (p = 0.002)。肿瘤 PCNA 指数较低的患者（3.5%，p = 0.019）具有显着的生存优势，非转移性疾病患者也存在差异（p = 0.012）。我们的数据表明 PCNA 表达可以进行分析，并且它可能构成肾细胞癌患者的额外预后参数。
The proliferating cell nuclear antigen (PCNA) is a nuclear protein associated with cells in active cell cycle. The expression of PCNA was evaluated immunohistochemically in 3-6 samples from 68 renal cell carcinomas. A total of 279 tumour samples were analysed using the monoclonal antibody PC-10, and the percentage of positively stained cell nuclei (PCNA-index) was determined. The tumour PCNA-index varied between 0.9-17.5%, with a mean value of 4.9%, significantly different from normal kidney cortex samples (p < 0.001). A significant difference in PCNA-index was found between the different tumour grades, whereas no significant difference was found between clinical stages. Concerning DNA ploidy, aneuploid tumours had a significantly higher PCNA-index compared with diploid tumours (p = 0.002). There was a significant survival advantage for patients with low tumour PCNA-indices (3.5%, p = 0.019), a difference also found for patients with non-metastatic disease (p = 0.012). Our data shows that PCNA expression can be analysed and that it may constitute an additional prognostic parameter fbr patients with renal cell carcinoma.