Epitope-tagged L* protein of Theiler's murine encephalomyelitis virus is expressed in the central nervous system in the acute phase of infection
Epitope-tagged L* protein of Theiler's murine encephalomyelitis virus is expressed in the central nervous system in the acute phase of infection
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DOI：
10.1128/jvi.76.24.13049-13054.2002
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发表时间：
2002-12-01
影响因子：
5.4
通讯作者：
Ohara, Y
中科院分区：
文献类型：
--
作者：
Asakura, K;Murayama, H;Ohara, Y
Theiler小鼠脑脊髓炎病毒（TMEV） TO亚群菌株通过一个替代起始密码子合成L*蛋白。我们首先用抗L*抗体免疫沉淀和免疫印迹法证实了L*在tmev感染小鼠急性期中枢神经系统（CNS）中的表达。此外，我们还生成了能够合成FLAG或3xFLAG表位标记的L*蛋白的突变病毒。我们用一种表达3xFLAG表位标记L*的突变病毒，命名为DA/3xFLAGL*，研究了感染急性期中枢神经系统中的L*。与野生型病毒相比，DA/3xFLAGL*没有改变病毒的嗜性，L*在易感和耐药小鼠的中枢神经系统中均有明显的表达。双免疫标记研究表明，L*与TMEV多蛋白共定位，并仅在神经元中表达。
TO subgroup strains of Theiler's murine encephalomyelitis virus (TMEV) synthesize L* protein from an alternative initiation codon. We first demonstrated L* expression in the central nervous system (CNS) of TMEV-infected mice during the acute phase of infection by immunoprecipitation and immunoblotting with anti-L* antibody. In addition, we generated mutant viruses which synthesize FLAG or 3xFLAG epitope-tagged L* protein. With a mutant virus expressing 3xFLAG epitope-tagged L*, designated DA/3xFLAGL*, we investigated L* in the CNS in the acute phase of infection. DA/3xFLAGL* did not change the virus tropism in comparison with wild-type virus, and L* was clearly identified in the CNS in both susceptible and resistant strains of mice. Double immunolabeling studies showed that L* is colocalized with TMEV polyprotein and exclusively expressed in neurons.