Calcium channel expression in the oligodendrocyte lineage
Calcium channel expression in the oligodendrocyte lineage
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DOI：
10.1111/j.1460-9568.1992.tb00130.x
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发表时间：
1992-01-01
影响因子：
3.4
通讯作者：
Kettenmann, Helmut
中科院分区：
文献类型：
--
作者：
Blankenfeld, Gabriela V.;Verkhratsky, Alexej N.;Kettenmann, Helmut
可以在体外研究少突胶质细胞从其前体细胞经过不同发育阶段的发育。这些阶段可以通过特异性单克隆抗体和特征性K+通道谱来区分。在这项研究中，我们表明，发生的Ca-2+电流也经历了显着的变化，在小鼠少突胶质细胞的发展。未成熟前体细胞可发育为星形胶质细胞或少突胶质细胞，它们表达两种不同类型的电压激活Ca-2+通道。前体细胞中Ca-2+通道的表达与Na+通道的表达密切相关。当细胞开始表达O 1抗原，并致力于少突胶质细胞系，Ca-2+和Na+电流不能再检测。大的Ca-2+电流，然而，记录在少突胶质细胞的发展，与O 10抗原的表达。根据其激活范围，Ca-2+通道被分类为高电压激活的Ca-2+通道和低电压激活的Ca-2+通道，并通过其动力学和药理学特性进一步描述。
The development of oligodendrocytes from their precursor cells through different developmental stages can be studied in vitro. These stages can be distinguished by specific monoclonal antibodies and by a characteristic K+ channel profile. In this study we demonstrate that the occurrence of Ca-2+ currents also undergoes marked changes during the development of mouse oligodendrocytes. Immature precursor cells which can develop into astrocytes or oligodendrocytes expressed two different types of voltage-activated Ca-2+ channels. The expression of Ca-2+ channels in precursor cells was strongly correlated with the expression of Na+ channels. When cells started to express the O1 antigen and were committed to the oligodendrocyte lineage, Ca-2+ and Na+ currents could no longer be detected. Large Ca-2+ currents were, however, recorded later in the development of the oligodendrocytes, correlated with the expression of the O10 antigen. The Ca-2+ channels were classified as high and low voltage-activated Ca-2+ channels according to their range of activation, and are further described by their kinetic and pharmacological properties.