Macropinocytosis is the endocytic pathway that mediates macrophage foam cell formation with native low density lipoprotein
Macropinocytosis is the endocytic pathway that mediates macrophage foam cell formation with native low density lipoprotein
复制标题
DOI：
10.1074/jbc.m407167200
复制
发表时间：
2005-01-21
影响因子：
4.8
通讯作者：
Zhang, WY
中科院分区：
文献类型：
--
作者：
Kruth, HS;Jones, NL;Zhang, WY
此前，我们曾报道PMA激活的人单核细胞来源的巨噬细胞对天然低密度脂蛋白的液态内吞作用将这些巨噬细胞转化为富含胆固醇的泡沫细胞(Kruth，H.S.，Huang，W.，Ishii，I.，and Zhang，W.Y.(2002)J.Biol)。化学。277、34573-34580)。细胞对液体的吸收可以通过囊泡内的微胞饮作用(是其在培养液中浓度的40倍)发生，这与大胞体成熟为多囊体体内体而收缩一致。巨噬细胞吞噬低密度脂蛋白是产生巨噬细胞泡沫细胞的一种新的内吞途径。巨噬细胞和其他血管细胞的巨噬细胞吞噬作用是调控动脉粥样硬化泡沫细胞形成的新途径。
Previously, we reported that fluid-phase endocytosis of native LDL by PMA-activated human monocyte-derived macrophages converted these macrophages into cholesterol-enriched foam cells (Kruth, H. S., Huang, W., Ishii, I., and Zhang, W. Y. ( 2002) J. Biol. Chem. 277, 34573 - 34580). Uptake of fluid by cells can occur either by micropinocytosis within vesicles ( 40-fold over its concentration in the culture medium consistent with macropinosome shrinkage by maturation into multivesicular body endosomes. Macropinocytosis of LDL taken up in the fluid phase without receptor-mediated binding of LDL is a novel endocytic pathway that generates macrophage foam cells. Macropinocytosis in macrophages and possibly other vascular cells is a new pathway to target for modulating foam cell formation in atherosclerosis.