PEG-variant biomaterials as selectively adhesive protein templates: model surfaces for controlled cell adhesion and migration
PEG-variant biomaterials as selectively adhesive protein templates: model surfaces for controlled cell adhesion and migration
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DOI：
10.1016/s0142-9612(99)00212-4
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发表时间：
2000-03-01
期刊：
影响因子：
14
通讯作者：
Moghe, PV
中科院分区：
文献类型：
--
作者：
Tziampazis, E;Kohn, J;Moghe, PV
我们的研究集中在聚乙二醇在主动调节蛋白质吸附生物材料的生物响应性方面的作用。为此，我们设计了一系列酪氨酸/聚乙二醇衍生聚碳酸酯中的聚乙二醇型生物材料，以呈现从低浓度到中等浓度的表面，低于完全消除蛋白质吸附所需的聚乙二醇水平。我们分析了聚乙二醇浓度对吸附的模型蛋白纤维连接蛋白的数量、构象和生物活性的影响，以及对L929成纤维细胞的黏附、黏附强度和运动能力的影响。我们的结果表明，低水平的聚乙二醇不仅可以调节吸附的纤维连接蛋白的程度，而且还可以调节纤维连接蛋白的构象和比生物活性。当聚乙二醇的浓度从0增加到6mol%时，纤维连接蛋白的吸附量呈线性下降，但纤维连接蛋白的构象发生了改变，从而使吸附的纤维连接蛋白的整体生物活性保持不变。我们报告了细胞附着程度随聚乙二醇浓度变化的方式，类似于纤维连接蛋白生物活性依赖于聚乙二醇组分。相比之下，细胞黏附强度依赖于聚乙二醇的性质与观察到的纤维连接蛋白表面浓度的模式平行。我们的研究还表明，在小范围的聚乙二醇浓度范围内，细胞的迁移速率与聚乙二醇浓度呈负相关。总体而言，这些结果突出了聚乙二醇型生物材料系统地调节吸附的细胞黏附蛋白的行为，从而影响细胞功能的惊人能力。(C)2000爱思唯尔科学有限公司。保留所有权利。
Our study focused on the role of poly(ethylene glycol) (PEG) in actively regulating the biological responsiveness of protein-adsorbed biomaterials. To this end, we designed PEG-variant biomaterials from a family of tyrosine/PEG-derived polycarbonates to present surfaces ranging from low to intermediate levels of PEG concentration, below the PEG level requisite for complete abolition of protein adsorption. We analyzed the effect of PEG concentration on the amount, conformation and bioactivity of an adsorbed model protein, fibronectin, and on the attachment, adhesion strength and motility of L929 fibroblasts. Our results demonstrate that low levels of PEG can regulate not only the extent but also the conformation and specific bioactivity of adsorbed fibronectin. As the PEG concentration was increased from 0 to 6 mol%, the amount of adsorbed fibronectin decreased linearly yet the fibronectin conformation was altered such that the overall bioactivity of adsorbed fibronectin was uncompromised. We report that the degree of cell attachment varied with PEG concentration in a manner similar to the dependence of fibronectin bioactivity on PEG. Tn contrast, the nature of cell adhesion strength dependence on PEG paralleled the pattern observed for fibronectin surface concentration. Our studies also indicated that the rate of cell migration was inversely correlated with PEG concentration over a narrow range of PEG concentration. Overall, these results highlight the striking ability of PEG-variant biomaterials to systematically regulate the behavior of adsorbed cell adhesion proteins and: consequently, effect cell functions. (C) 2000 Elsevier Science Ltd. All rights reserved.