High-fat diet increases labial groove formation in maxillary incisors and is related to aging in C57BL/6 mice
High-fat diet increases labial groove formation in maxillary incisors and is related to aging in C57BL/6 mice
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DOI：
10.1016/j.job.2019.12.003
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发表时间：
2020-03-01
影响因子：
2.4
通讯作者：
Ota, Masato S.
中科院分区：
文献类型：
--
作者：
Imai, Atsuko;Yamashita, Atsuko;Ota, Masato S.
目的：本研究的目的是探讨 C57BL/6 小鼠高脂饮食的摄入与年龄依赖性上颌切牙沟形成之间的关系，并鉴定推定的上颌切牙沟相关基因。方法：我们用普通饮食或高脂饮食喂养 2 个月大和 16 个月大的 C57BL/6 小鼠三个月，观察变化 位于上颌切牙沟内。我们检查了有凹槽的上颌切牙的组织切片，并对有/无凹槽的上颌切牙的根尖组织碎片进行了转录组分析。结果：三个月的高脂肪饮食导致体重和切牙凹槽数量显着增加。从 26 个月到 28 个月，切牙凹槽的数量和频率都以与年龄相关的方式增加，在此期间出现了额外的凹槽。上颌切牙沟唇面成釉细胞异常分化和凋亡。转录组分析确定了 23 个 24 个月大小鼠特有的基因；其中包括与细胞凋亡和细胞分化相关的几个基因。结论：研究结果表明，在C57BL/6小鼠中，食用高脂肪饮食会增加上颌切牙的唇沟形成，这与牙齿根尖部组织干细胞的老化有关。 (C) 2019 年日本口腔生物学协会。由 Elsevier B.V. 出版。保留所有权利。
Objectives: The aim of this study was to explore the relationship between the consumption of a high-fat diet and aging-dependent formation of maxillary incisor grooves in C57BL/6 mice, and to identify putative maxillary incisor groove-related genes.Methods: We fed 2-month-old and 16-month-old C57BL/6 mice on either a chow diet or a high-fat diet for three months and observed changes in maxillary incisor grooves. We examined tissue sections of the maxillary incisors with grooves and carried out transcriptome analysis of the apical tissue fragments of maxillary incisors with/without grooves.Results: Consumption of a high-fat diet for three months resulted in significant increases in both body weight and the number of incisor grooves. Both the number and frequency of incisor grooves increased in an age-dependent manner from 26 to 28 months, during which time an additional groove appeared. There was abnormal differentiation and apoptosis of ameloblasts on the labial surface at the grooves of the maxillary incisors. Transcriptome analysis identified 23 genes as being specific to 24-month-old mice; these included several genes related to apoptosis and cell differentiation.Conclusions: The study findings indicate that, in C57BL/6 mice, consumption of a high-fat diet increases labial groove formation in maxillary incisors, which is related to aging of the tissue stem cells in the apical root end of the teeth. (C) 2019 Japanese Association for Oral Biology. Published by Elsevier B.V. All rights reserved.