Oligosaccharide-Carrying Styrene-Type Macromers. Polymerization and Specific Interactions Between the Polymers and Liver Cells
Oligosaccharide-Carrying Styrene-Type Macromers. Polymerization and Specific Interactions Between the Polymers and Liver Cells
复制标题
携带寡糖的苯乙烯型大分子单体。
DOI：
10.1080/00222338808053391
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发表时间：
1988
期刊：
影响因子：
--
通讯作者：
T. Akaike
中科院分区：
文献类型：
--
作者：
Kazukiyo Kobayashi;H. Sumitomo;A. Kobayashi;T. Akaike
摘要采用对乙烯基苄胺偶联低聚糖内酯合成的一类新型大分子，通过自由基聚合法制备了各苯环上含有乳糖、葡萄糖、麦芽糖、麦芽糖三糖、麦芽糖戊二糖和麦芽糖七糖的聚苯乙烯衍生物。这些由两亲结构单元组成的聚合物具有水溶性，有机溶质在水中与聚合物的疏水微环境结合。携带α- d - glucopyranse的聚合物被concanavalin a识别并沉淀，肝细胞（肝细胞）的培养尝试使用表面涂有携带乳糖、葡萄糖、麦芽糖和麦芽糖的聚苯乙烯的培养皿。研究发现，携带乳糖的聚苯乙烯（PVLA）是一种有用的肝细胞培养表面材料。1)在添加或不添加血清的pvlaa包被培养皿中获得了肝细胞的高度特异性粘附。2)细胞黏附是一种阈值现象。
Abstract Polystyrene derivatives with lactose, glucose, maltose, maltotriose, maltopentaose, and maltoheptaose on each benzene ring were prepared by the radical polymerization of a new class of macromers synthesized by coupling the corresponding oligosaccharide lactones with p-vinylbenzylamine. These polymers consisting of amphiphilic structural units were water-soluble, and organic solutes were bound to hydrophobic microenvironments of the polymers in water. α-D-Glucopyranose-carrying polymers were recognized and precipitated by concanavalin A. Cultivation of liver cells (hepatocytes) was attempted using culture dishes whose surface was coated with lactose-, glucose-, maltose-, and maltotriose-carrying polystyrenes. It has been found that a lactose-carrying polystyrene (PVLA) is a useful surface material for hepatocyte culture. 1) Highly specific adhesion of hepatocytes was attained for PVLA-coated dishes with or without serum supplement. 2) The cell adhesion was a threshold phenomenon with respect to th...
影响因子：
2.9
作者：
LEE, RT;LIN, P;LEE, YC
通讯作者：
LEE, YC