A study of astrocyte activation in the periinfarct region after cerebral ischemia with electroacupuncture
A study of astrocyte activation in the periinfarct region after cerebral ischemia with electroacupuncture
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DOI：
10.3109/02699051003610482
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发表时间：
2010-05-01
期刊：
影响因子：
1.9
通讯作者：
Chen, Hong
中科院分区：
文献类型：
--
作者：
Han, Xiaohua;Huang, Xiaolin;Chen, Hong
目标：目的：探讨电针对脑缺血后梗死灶周围区星形胶质细胞（AST）活化的影响，以及电针治疗后行为学的改善作用。每组大鼠又分为7、14和28天亚组。建立大鼠大脑中动脉闭塞模型，电针“百会”、“大椎”穴7、14、28天。GFAP免疫反应阳性细胞的数量进行了测定和行为测试performed.Results：许多更多的GFAP免疫反应阳性细胞检测到在EA组在第14天比在缺血后第7天和免疫反应阳性细胞的数量略有下降，观察到在第28天。电针组动物的行为学测试结果在平衡木平衡能力和牵拉能力上均优于模型组动物，且具有上级作用。结论：电针具有激活梗死周围区AST的潜力，可避免过度的反应性胶质增生，并可促进缺血后行为功能障碍的恢复。
Objective: To investigate the effects of electroacupuncture (EA) on the activation of astrocytes (AST) in the periinfarct region after cerebral ischemia, as well as to evaluate potential behavioural improvement after EA treatment.Methods: Male Wistar rats were randomly divided into normal, model and EA groups. Rats in each group were further divided into 7, 14 and 28 days sub-groups. A model of middle cerebral artery occlusion was established, followed by 7, 14 or 28 days of EA on 'Bai hui' and 'Da zhui' acupoints. The number of GFAP-immunoreactive cells was determined and behavioural tests were performed.Results: Many more GFAP-immunoreactive cells were detected in the EA group on day 14 than on day 7 after ischemia and a slightly decreased number of immunoreactive cells were observed on day 28. The results of behavioural tests of animals in the EA group on the beam balance performance and prehensile traction performance were superior to that of animals in the model group.Conclusion: These findings indicate that EA has the potential to activate AST in the periinfarct region and to avoid excess reactive gliosis and also can facilitate the recovery of post-ischemic behavioural dysfunction.