Amitosis of Polyploid Cells Regenerates Functional Stem Cells in the Drosophila Intestine.
Amitosis of Polyploid Cells Regenerates Functional Stem Cells in the Drosophila Intestine.
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DOI：
10.1016/j.stem.2017.02.012
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发表时间：
2017-05-04
期刊：
影响因子：
23.9
通讯作者：
Ohlstein B
中科院分区：
文献类型：
--
作者：
Lucchetta EM;Ohlstein B
Organ fitness depends on appropriate maintenance of stem cell populations, and aberrations in functional stem cell numbers are associated with malignancies and aging. Symmetrical division is the best characterized mechanism of stem cell replacement, but other mechanisms could also be deployed, particularly in situations of high stress. Here, we show that after severe depletion, intestinal stem cells (ISCs) in the Drosophila midgut are replaced by spindle-independent ploidy reduction of cells in the enterocyte-lineage through a process known as amitosis. Amitosis is also induced by the functional loss of ISCs coupled with tissue demand and in aging flies, underscoring the generality of this mechanism. However, we also found that random homologous chromosome segregation during ploidy reduction can expose deleterious mutations through loss of heterozygosity. Together, our results highlight amitosis as an unappreciated mechanism for restoring stem cell homeostasis, but one with some associated risk in animals carrying mutations.
