SCHISTOSOMA-MANSONI - EFFECT OF TRANSFERRIN AND GROWTH-FACTORS ON DEVELOPMENT OF SCHISTOSOMULA INVITRO
SCHISTOSOMA-MANSONI - EFFECT OF TRANSFERRIN AND GROWTH-FACTORS ON DEVELOPMENT OF SCHISTOSOMULA INVITRO
复制标题
DOI：
10.2307/3282599
复制
发表时间：
1989-06-01
影响因子：
1.3
通讯作者：
BASCH, PF
中科院分区：
文献类型：
--
作者：
CLEMENS, LE;BASCH, PF
我们研究了人转铁蛋白和外源铁对曼氏血吸虫体外早期生长和发育的作用，通过发育成就和胸苷掺入来确定。为了帮助确定适当的无血清培养基，我们还利用了成纤维细胞生长因子、血小板衍生生长因子、表皮生长因子和增殖刺激活性，所有这些都存在于血吸虫发育的肝脏环境中，以及某些商业血清替代品。基础培养基中添加250-1,000μg/ml范围内的转铁蛋白提供了等于或优于添加血清的培养基的发育，至少在培养的前2周内如此。补充有 Nutridoma-HU 或 -SP 的无血清培养基在较小程度上刺激发育。铁（如硫酸铁）对胸苷掺入的刺激作用可被去铁胺（一种螯合剂）消除。用 125I 标记和未标记的转铁蛋白进行的竞争性抑制研究表明，血吸虫上存在不饱和结合位点。
We have studied the role of human transferrin and of exogenous iron on early growth and development of schistosomula of Schistosoma mansoni in vitro, as determined by developmental achievement and thymidine incorporation. To help define an adequate serumless medium we also utilized fibroblast growth factor, platelet-derived growth factor, epidermal growth factor, and multiplication-stimulating activity, all present in the hepatic environment of developing schistosomula, as well as certain commercial serum substitutes. Supplementation of basal medium with transferrin in the range of 250-1,000 .mu.g/ml provided development equal or superior to serum-supplemented medium, at least within the first 2 wk of culture. Serum-free medium supplmented with Nutridoma-HU or -SP stimulated development to a lesser extent. The stimulatory effect of iron (as ferric sulfate) on thymidine incorporation was removed by deferroxamine, a chelating agent, Competitive inhibition studies with 125I-labeled and unlabeled transferrin indicated the presence of nonsaturable binding sites on schistosomula.