Enhancement of liver microsome epoxide hydratase activity in rodents by treatment with 2(3)-tert-butyl-4-hydroxyanisole.
Enhancement of liver microsome epoxide hydratase activity in rodents by treatment with 2(3)-tert-butyl-4-hydroxyanisole.
复制标题
通过 2(3)-叔丁基-4-羟基苯甲醚处理增强啮齿动物肝微粒体环氧化物水合酶活性。
作者：
Y. Cha;F. Martz;E. Bueding
日粮中加入抗氧化剂2(3)-叔丁基-4-羟基苯甲醚(BHA)可显著提高CD-1小鼠肝微粒体环氧化物水合酶(EC 4.2.1.63)的比活力。BHA产生的环氧化物水合酶活性的增加(11倍)远远大于使用众所周知的酶诱导剂，如3-甲基胆蒽、苯巴比妥和Aroclor 1254(2-3倍)。在饲喂BHA饲料3天后，观察到环氧化物水合酶活性几乎最大限度地增加。当BHA经胃管给药时，增加的水平仅为饲料中BHA的75%。BHA处理的SD大鼠产生的环氧化物水合酶活性的增加不像CD-1小鼠那样明显(不到3倍)。
Administration of the antioxidant 2(3)-tert-butyl-4-hydroxyanisole (BHA) in the diet caused a marked increase in the specific activity of epoxide hydratase (EC 4.2.1.63) in hepatic microsomes of CD-1 mice. The increases in epoxide hydratase activities produced by BHA were far greater (11-fold) than were those produced by the administration of well-known enzyme inducers such as 3-methylcholanthrene, phenobarbital, and Aroclor 1254 (2- to 3-fold). The near-maximal increase in epoxide hydratase activity was observed after feeding of the BHA diet for 3 days. When BHA was administered by gastric intubation, the level of increase was only 75% of that attained by feeding BHA in the diet. The increase in epoxide hydratase activity produced by BHA treatment of Sprague-Dawley rats was not as pronounced (less than 3-fold) as that observed in CD-1 mice.